Quantitation of some Pancreatic Juice Exocrine Proteins in the Presence of Radiocontrast Medium used in Endoscopic Retrograde Pancreatography (ERP)

Summary

The endoscopic collection of human pancreatic juice for chemical analysis is facilitated by the use of radiocontrast media. However, the effects of this material on pancreatic juice and the techniques used to analyze its proteins are unknown. This study has demonstrated no detrimental effects of a commonly used contrast medium on the pancreatic juice itself or on the analytic technique of radioimmunoassay and radial immunodiffusion. Likewise, the Phadebas enzymatic technique for amylase was not affected by this material. We conclude that techniques for the collection of pure pancreatic juice using radiocontrast material do not interfere with the analysis of pancreatic juice proteins using the techniques described.

Key words:

Amylase - Cholecystokinin - Pancreatic secretin - Secretion trypsin - Trypsin inhibitors