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Thromb Haemost 1996; 75(05): 772-777
DOI: 10.1055/s-0038-1650365
Original Article
Schattauer GmbH Stuttgart

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

Sybille Albrecht
The Institut für Pathologie, Technische Universitat Dresden, Germany
,
Matthias Kotzsch
The Institut für Pathologie, Technische Universitat Dresden, Germany
,
Gabriele Siegert
1   Institut für Klinische Chemie und Laboratoriumsmedizin, Technische Universitat Dresden, Germany
,
Thomas Luther
The Institut für Pathologie, Technische Universitat Dresden, Germany
,
Heinz Großmann
The Institut für Pathologie, Technische Universitat Dresden, Germany
,
Marianne Großer
The Institut für Pathologie, Technische Universitat Dresden, Germany
,
Martin Müller
The Institut für Pathologie, Technische Universitat Dresden, Germany
› Author Affiliations
Further Information

Publication History

Received 20 July 1995

Accepted after resubmission 05 February 1996

Publication Date:
10 July 2018 (online)

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Summary

The plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

 

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