Identification and Biosynthesis of Fibrinogen in Human Uterine Cervix Carcinoma Cells

Summary

Fibrinogen has been detected in ME-180 human uterine cervix carcinoma cells, and synthesis of fibrinogen by ME-180 cells has been measured using [³⁵S] L-methionine incorporation. Expression of mRNA for the B|3-chain of the fibrinogen in ME-180 cells has been identified by PCR-assisted mRNA amplification. The AΑ-, BΒ-, and Γ-chains of fibrinogen synthesized by ME-180 cells were chemically and immunologically identical to those of plasma fibrinogen. These findings suggest that fibrinogen is present and synthesized in ME-180 human uterine cervix carcinoma cells, and that fibrinogen plays a role in these malignant tumor cells.

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