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DOI: 10.1055/s-2000-9876
Peroxidase Activity in Aloe barbadensis Commercial Gel: Probable Role in Skin Protection
Publikationsverlauf
Publikationsdatum:
31. Dezember 2000 (online)
Abstract
A basic peroxidase (EC 1.11.1.7) (pI around 9.0) has been identified in commercial gel of Aloe barbadensis. In vivo, the activity is localised in the vascular system of inner aqueous leaf parenchyma. Some relevant properties of this basic peroxidase of Aloe have been investigated in leaf extract and in commercial gel where it is notably stable. The acid optimum pH (5.0) for activity and the low K M for H2O2 (0.14 mM) suggest that, when topically applied, Aloe peroxidase may scavenge H2O2 in skin surface.
Abbreviations
4-MN:4-methoxy-α-naphthol BSA:bovine serum albumin EGTA:ethylene glycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid HQ:p-hydroquinone IEF:isoelectrofocusing IALP:inner aqueous leaf parenchyma PVDF:polyvinylidene difluoride ROS:reactive oxygen species pI:isoelectric pH
Key words
Ageing - Aloe barbadensis - Asphodelaceae - peroxidase - reactive oxygen species - skin photo-damage
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Dr. Bartolomé Sabater
Departamento de Biología Vegetal Universidad de Alcalá
Alcalá de Henares
28871 Madrid
Spain
eMail: bvsabater@alcala.es
Telefon: +34 91 885 5066
Fax: +34 91 885 4934