New Synthetic Models of Cytochrome P450: How Different Are They from the Natural Species?

Abstract

Soluble and matrix-bound P450 enzyme models have been synthesized carrying a SO₃ ^- ligand coordinating to iron. These complexes display features very similar to cofactors of enzymes such as P450_cam with respect to electrochemistry and UV/Vis spectroscopy. Further they catalyze epoxidation reactions with turnover numbers up to 1800. DFT calculations revealed that the coordination of SO₃ ^- to Fe(III) produces an active species that displays allylic hydroxylation and epoxidation reactivity patterns that are nearly indistinguishable from those calculated for the natural active species of the enzyme cytochrome P450.

Key words

catalysis - enzyme models - DFT calculations - heme-thiolate proteins - iron porphyrins

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