Genetic Variation in Wild and Cultivated Rhizoma Corydalis Revealed by ISSRs Markers

Abstract

Rhizoma Corydalis is an important Chinese medicinal herb. In this paper, we employed ISSR data to explore the genetic variation in domesticated populations and wild populations of the species. The average of within-population ISSR diversity in cultivated populations (PPF = 25.32 %, Hpop = 0.094) was lower than that in wild populations (PPF = 47.70 %, Hpop = 0.144). Cultivated populations (Φ _ST = 0.515, G _ST = 0.429) have a greater proportion of their genetic variability distributed among populations than wild populations (Φ _ST = 0.277, G _ST = 0.226). Based on hierarchical estimates of variance components, significant statistical differences (57.77 %, P < 0.001) were found between the wild and cultivated groups. The low levels of genetic diversity within cultivated populations and high levels of genetic differentiation among populations/groups may result from artificial selection, the mode of clonal propagation, and only limited exchange of material among localities. Finally, some suggestions for conservation and efficient management of the genetic resources of this important medicinal herb are proposed.

Abbreviations

G _ST:Nei’s coefficient of population differentiation

Hpop:Nei’s expected heterozygosity

PPF:percentage of polymorphic fragments

Φ _ST:genetic differentiation estimated by AMOVA

Corydalis yanhusuo - Fumariaceae - traditional Chinese medicine - cultivated populations - wild populations - genetic variation - ISSR

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