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DOI: 10.1055/s-0028-1088365
© Georg Thieme Verlag KG Stuttgart · New York
Genetic Variation in Wild and Cultivated Rhizoma Corydalis Revealed by ISSRs Markers
Publication History
Received: April 6, 2008
Revised: August 4, 2008
Accepted: September 12, 2008
Publication Date:
25 November 2008 (online)
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Abstract
Rhizoma Corydalis is an important Chinese medicinal herb. In this paper, we employed ISSR data to explore the genetic variation in domesticated populations and wild populations of the species. The average of within-population ISSR diversity in cultivated populations (PPF = 25.32 %, Hpop = 0.094) was lower than that in wild populations (PPF = 47.70 %, Hpop = 0.144). Cultivated populations (Φ ST = 0.515, G ST = 0.429) have a greater proportion of their genetic variability distributed among populations than wild populations (Φ ST = 0.277, G ST = 0.226). Based on hierarchical estimates of variance components, significant statistical differences (57.77 %, P < 0.001) were found between the wild and cultivated groups. The low levels of genetic diversity within cultivated populations and high levels of genetic differentiation among populations/groups may result from artificial selection, the mode of clonal propagation, and only limited exchange of material among localities. Finally, some suggestions for conservation and efficient management of the genetic resources of this important medicinal herb are proposed.
Abbreviations
G ST:Nei’s coefficient of population differentiation
Hpop:Nei’s expected heterozygosity
PPF:percentage of polymorphic fragments
Φ ST:genetic differentiation estimated by AMOVA
Key words
Corydalis yanhusuo - Fumariaceae - traditional Chinese medicine - cultivated populations - wild populations - genetic variation - ISSR
- Supporting Information for this article is available online at
- Supporting Information .
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Prof. Cheng-Xin Fu
Laboratory of Systematic and Evolutionary Botany
Department of Biology
College of Life Sciences
Zhejiang University
Hangzhou 310058
People′s Republic of China
Phone: +86-571-8820-6607
Fax: +86-571-8643-2273
Email: cxfu@zju.edu.cn
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