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2 The lack of a stereogenic centre at
C-5 means that the stereochemistry of valienamine is described by
analogy to a pentose rather than as a hexose, hence it has the α-xylo configuration. 1-epi-Valienamine
has a β-xylo configuration,
and 2-epi-valienamine has an α-lyxo configuration. Valienamine derivatives
are numbered with carbasugar numbering to stress the homomorphic relationship
with carbohydrates.
3
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Mitsunobu Coupling: α-Alcohol 1 (54 mg, 0.10 mmol) and nosylamide 4 (65 mg, 0.10 mmol) were dissolved in
anhyd THF (2 mL) and the solution was cooled to 0 ˚C under
N2. PPh3 (53 mg, 0.202 mmol) was added, then
after 15 min, DIAD (39 µL, 0.198 mmol) was added dropwise.
After 30 min, the ice-bath was removed, and the reaction was allowed to
proceed at r.t. After TLC (pentane-EtOAc, 2:1) indicated the
consumption of the alcohol (R
f
0.2) and the sulfonamide (R
f
0.1)
and the formation of a product (R
f
0.15), the mixture was concentrated
in vacuo. The residue was purified by flash column chromatography
to give the β-linked pseudodisaccharide 9 (65
mg, 64%).
17 The stereochemistry of the bromides
(7 and 8) and pseudodisaccharides
(9, 10 and 12) was assigned using the J
1,2 and J
1,5a coupling
constants from the ¹H NMR spectra, in comparison
with reported data.²8 In α-xylo compounds, J
1,5a was
in the range 4.4-5.8 Hz; J
1,2 was
in the range 3.5-4.4 Hz. In β-xylo compounds,
H-5a appeared as a singlet; J
1,2 was between
7.1-9.2 Hz (not determined for 9).
These J
1,2 values are consistent
with the ²
H
3 half-chair
conformation expected for both diastereomers. For the nosylated pseudodisaccharide 9, many of the NMR resonances were broad,
possibly resulting from steric crowding and conformational change,
which weakens an argument for configurational assignment of this
compound based on coupling constants, but its configuration was
confirmed by its deprotection (PhSH, K2CO3,
DMF, 83%) to give 12.
18
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20
Palladium-Catalysed
Coupling: β-Imidate 6 (94
mg, 0.14 mmol), trimethylolpropane phosphite (TMPP; 5 mg, 0.028 mmol)
and Pd2(dba)3 (7 mg, 0.014 mmol) were placed
in a round-bottomed flask with a magnetic stirrer bar. The flask was
evacuated, then placed under Ar. Amine 3 (194
mg, 0.42 mmol) was suspended in MeCN (it was partially soluble), the
solvent was degassed and the mixture was placed under Ar. The amine
suspension was transferred to the reaction vessel by syringe. Et3N
(0.09 mL, 0.63 mmol) was added, and the mixture colour changed from
a purple/brown suspension to a pale yellow solution. The
reaction mixture was stirred at r.t. under Ar. After 15 h, TLC (pentane-EtOAc,
3:1) showed complete consumption of the imidate (R
f
0.8), amine remaining (R
f
0),
and the formation of a major product (R
f
0.1). The mixture was concentrated
in vacuo and the residue was purified by flash column chromatography
to give the β-linked pseudodisaccharide 12 (115
mg, 85%).
21a
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23
Coupling with
C-1 Bromides: β-Bromide 8 (42
mg, 0.070 mmol) and amine 3 (80 mg, 0.17
mmol) were dissolved in MeCN (1 mL) under Ar. N,N′-Diisopropylethylamine (35 µL,
0.20 mmol) was added and the reaction mixture was heated to 50 ˚C.
After 24 h, TLC (pentane-EtOAc, 2:1) indicated the formation
of a major product (R
f
0.3). The mixture was concentrated
in vacuo, and the residue was purified by flash column chromatography
to give the α-linked pseudodisaccharide 10 (46
mg, 67%).
24 The β-bromide 8 does
react at a higher rate with the amine 3 (reacting
slowly at r.t.) than does its α epimer 7 (which needed
heating for any reaction to take place).
25
Representative
Data:
2,3,4,6-Tetra-
O
-benzyl-5a-carba-β-
d
-
xylo
-hex-5(5a)-enopyranosyl
Trichloroacetimidate (6): colourless oil; [α]D
²¹ -53.9
(c = 1.0, CHCl3).
IR (film): 1662 (C=N) cm-¹. ¹H
NMR (500 MHz, CDCl3): δ = 3.91-4.00
(m, 3 H, H-2, H-3, H-6), 4.23 (d, J
6,6
′ = 12.2
Hz, 1 H, H-6′), 4.37 (d, J
3,4 = 7.5 Hz,
1 H, H-4), 4.49, 4.53 (2 × d, J = 11.9
Hz, 2 H, PhCH
2), 4.73 (d, J = 10.9 Hz, 1 H, PhCHH′), 4.82, 5.00 (2 × d, J = 11.0 Hz, 2 H, PhCH
2), 4.85-4.91 (m,
3 H, PhCH
2, PhCHH′), 5.75 (d, J
1,2 = 7.1
Hz, 1 H, H-1), 5.82 (s, 1 H, H-5a), 7.26-7.35 (m, 20 H,
ArH), 8.48 (s, 1 H, NH). ¹³C NMR (125
MHz, CDCl3): δ = 69.7, 72.5, 74.8,
75.3, 75.5, 79.5, 79.6, 81.7, 83.9, 91.4, 122.1, 127.6, 127.7, 127.7,
127.8, 127.9, 127.9, 128.3, 128.4, 128.4, 137.9, 138.2, 138.3, 138.4,
138.6, 162.1. HRMS-ESI: m/z [M + Na]+ calcd
for C37H36O5NCl3Na:
702.1551; found: 702.1521.
2,3,4,6-Tetra-
O
-benzyl-5a-carba-β-
d
-
xylo
-hex-5(5a)-enopyranosyl
Bromide (8): colourless oil; [α]D
²³ -73.3
(c = 1.0, CHCl3). ¹H
NMR (500 MHz, CDCl3): δ = 3.75 (dd,
J
2,3 = 10.2
Hz, J
3,4 = 8.0 Hz,
1 H, H-3), 3.94-3.98 (m, 2 H, H-2, H-6), 4.23 (d, J
6,6
′ = 12.7
Hz, 1 H, H-6′), 4.37 (d, J
3,4 = 8.0
Hz, 1 H, H-4), 4.51, 4.54 (2 × d, J = 11.8
Hz, 2 H, PhCH
2), 4.73 (d, J = 10.8 Hz, 1 H, PhCHH′), 4.76 (d, J
1,2 = 7.9
Hz, 1 H, H-1), 4.82 (d, J = 11.0
Hz, 1 H, PhCHH′), 4.86 (d, J = 10.9 Hz, 1 H, PhCHH′), 4.93-4.98 (m,
3 H, PhCH
2, PhCHH′), 5.91 (s, 1 H, H-5a), 7.25-7.41
(m, 20 H, ArH). ¹³C NMR (125 MHz, CDCl3): δ = 51.7,
69.5, 72.6, 75.4, 75.7, 76.2, 79.8, 85.0, 85.5, 125.9, 127.9, 127.9,
127.9, 127.9, 128.0, 128.0, 128.3, 128.5, 128.5, 128.6, 137.7, 138.1, 138.1,
138.3, 138.4. HRMS-ESI: m/z [M + Na]+ calcd
for C35H35O4BrNa: 621.1611; found:
621.1610.
Methyl 6-[2,3,4,6-Tetra-
O
-benzyl-5a-carba-β-
d
-
xylo
-hex-5(5a)-enopyranosylamino]-2,3,4-tri-
O
-benzyl-6-deoxy-α-
d
-glucopyranoside
(12): white solid; [α]D
²5 -21.2 (c = 1.0, CHCl3). ¹H
NMR (500 MHz, CDCl3): δ = 2.60 (dd, J
5,6 = 6.4 Hz, J
6,6
′ = 11.7
Hz, 1 H, H-6I), 3.01 (dd, J
5,6
′ = 2.5 Hz, J
6,6
′ = 11.7
Hz, 1 H, H-6′I), 3.29 (s, 3 H, OMe), 3.36 (m, 1
H, H-1II), 3.46-3.51 (m, 2 H, H-2I,
H-4I), 3.59 (at J = 9.2 Hz,
1 H, H-2II), 3.73 (m, 1 H, H-5I), 3.87-3.90
(m, 2 H, H-3II, H-6II), 3.99 (at, J = 9.3 Hz, 1 H, H-3I),
4.26 (d, J
6,6
′ = 12.0 Hz,
1 H, H-6′II), 4.33 (d, J
3,4 = 7.1
Hz, 1 H, H-4II), 4.41-5.00 (m, 15 H, 7 × PhCH
2, H-1I), 5.67
(s, 1 H, H-5aII), 7.23-7.40 (m, 35 H, ArH). ¹³C
NMR (125 MHz, CDCl3): δ = 47.1, 55.3,
60.0, 70.3, 70.5, 72.3, 73.5, 74.7, 75.1, 75.2, 75.4, 75.9, 79.3,
80.2, 80.4, 82.0, 82.1, 85.2, 98.1, 127.2, 127.7, 127.8, 127.9,
127.9, 128.0, 128.1, 128.3, 128.5, 128.5, 128.5, 128.6, 135.6, 138.4,
138.4, 138.6. HRMS-ESI: m/z [M + Na]+ calcd
for C63H67NO9Na: 1004.4708; found:
1004.4626.
26 Acarbose is used as a diabetes type
2 drug; Validamycin A is used as an agrochemical fungicide against
rice sheath blight.
27
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