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Planta Med 2011; 77(13): 1519-1524
DOI: 10.1055/s-0030-1270743
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic and Antimicrobial Activities of Aporphine Alkaloids Isolated from Stephania venosa (Blume) Spreng

Arthit Makarasen1 , Wandee Sirithana2 , Samang Mogkhuntod2 , Nisachon Khunnawutmanotham1 , Nitirat Chimnoi1 , Supanna Techasakul1 , 2
  • 1Chulabhorn Research Institute, Bangkok, Thailand
  • 2Department of Chemistry, The Center of Innovation in Chemistry (PERCH‐CIC), Kasetsart University, Bangkok, Thailand
Further Information

Publication History

received October 10, 2010 revised January 5, 2011

accepted January 15, 2011

Publication Date:
08 February 2011 (online)

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Abstract

The cytotoxic activity of five alkaloids, namely 4,5-dioxo-dehydrocrebanine (1), dehydrocrebanine (2), crebanine (3), oxostephanine (4), and thailandine (5) isolated from the tuber and leaves of Stephania venosa (Blume) Spreng was investigated. Thailandine showed the strongest activity against lung carcinoma cells (A549) (IC50 of 0.30 µg/mL) with very low cytotoxicity against normal embryonic lung cells (MRC-5). Thailandine also demonstrated strong activity against Plasmodium falciparum, K1 strain (IC50 of 20 ng/mL), and Mycobacterium tuberculosis H37Ra (MIC of 6.25 µg/mL) as well as gram-positive bacteria such as Streptococcus pneumoniae and Staphylococcus aureus. Oxostephanine exhibited strong activity against breast cancer (BC) and acute lymphoblastic leukemia cells (MOLT-3) with an IC50 of 0.24 and 0.71 µg/mL, respectively, and exhibited very low cytotoxicity against MRC-5 cells. Dehydrocrebanine demonstrated strong activity against promyelocytic leukemia cells (HL-60) with an IC50 of 2.14 µg/mL whereas crebanine showed weak activity against cancer cell lines. However, both of them showed cytotoxicity against MRC-5 cells.

Key words

Stephania venosa (Blume) Spreng - Menispermaceae - thailandine - cytotoxicity - antimicrobial - antimalarial - antifungal

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Associate Professor Dr. Supanna Techasakul

Department of Chemistry
The Center of Innovation in Chemistry (PERCH-CIC)
Kasetsart University

50 Phaholyothin Road, Chatuchak

Bangkok 10900

Thailand

Phone: +66 25 74 06 22 13 06

Fax: +66 25 74 06 22 13 07

Email: fscispt@ku.ac.th

    www.thieme-connect.de/ejournals/toc/plantamedica