Leptin, Cortisol and Distinct Concurrent Training Sequences

Abstract

In order to investigate the effects of distinct concurrent training sequences on serum leptin and cortisol levels, 10 subjects (27.1±4.8 years, body mass index 25.38±0.09) were submitted to a control session, concurrent training 1 and concurrent training 2. Samples of leptin and cortisol were collected. Concurrent training 1 consisted of indoor cycling followed by strength training and concurrent training 2 of strength training followed by indoor cycling. No exercises were performed at the control session. Blood was collected once again to verify the same variables. Shapiro-Wilk, 2-way ANOVA and Tukey post-hoc tests were used. There was a reduction in leptin levels after concurrent training 1 (Δ%= − 16.04; p=0.05) and concurrent training 2 (Δ%= − 8.54; p=0.02). Cortisol decreased after concurrent training 1 (Δ%= − 26.32; p=0.02) and concurrent training 2 (Δ%= − 33.57; p=0.05). There was a high and significant correlation between blood variables only in CS (lep PRE X cort PRE and cort POST: r= − 0.80 and r= − 0.81; lep POST X cort PRE and cort POST: r= − 0.62 and r= − 0.62). Concurrent training promoted a reduction in leptin and cortisol levels irrespective of sequence.

Key words

hormones - physical exercise - aerobic exercise - strength exercise

• References

• 1 ACSM’s Guidelines For Exercise Testing And Prescription 7^th ed. Lippincott Williams & Wilkins; 2006
• 2 Baechle TR, Earle RW. Essencials of strength training and conditioning. Champaign: Human Kinetics; 2000
• 3 Bell GJ, Syrotuik D, Martin TP, Burnhan R, Quinney HA. Effect of concurrent strength and endurance training on skeletal muscle properties and hormone concentrations in humans. Eur J Appl Physiol 2000; 81: 418-427
• 4 Bornstein SR, Uhlmann K, Haidan A, Scherbaum WA. Evidence for a novel peripheral action of leptin as a metabolic signal to the adrenal gland: leptin inhibits cortisol release directly. Diabetes 1997; 46: 1235-1238
• 5 Cadore EL, Pinto R, Lhullier FLR, Correa C, Alberton C, Pinto S, Almeida APV, Tartaruga MP, Silva EM, Kruel LFM. Physiological effects of concurrent training in elderly men. Int J Sports Med 2010; 31: 689-697
• 6 Canali ES, Kruel LF. Respostas hormonais ao exercício. Rev Paul Educ Fís 2001; 15: 141-153
• 7 Dagogo-Jack S, Tykodi G, Umamaheswaran I. Inhibition of cortisol biosynthesis decreases circulating leptin levels in obese humans. J Clin Endocr Metab 2005; 90: 5333-5335
• 8 Wabitsch M, Jensen PB, Blum W, Christoffersen C, Englaro P, Heinze E. Insulin and cortisol promote leptin production in cultured human fat cells. Diabetes 1996; 45: 1435-1438
• 9 De Salles B, Simão R, Fleck S, Dias I, Kraemer-Aguiar L, Bouskela E. Effects of resistance training on cytokines. Int J Sports Med 2010; 31: 441-450
• 10 Duclos M, Gouarne C, Bonnemaison D. Acute and chronic effects of exercise on tissue sensitivity to glucocorticoids. J Appl Physiol 2003; 94: 869-875
• 11 França SCA, Barros-Neto TL. Resposta Divergente da Testosterona e do Cortisol Séricos em Atletas Masculinos Após Uma Corrida de Maratona. Arq Bras Endocrinol Metab 2006; 50: 1082-1087
• 12 Hackney AC, Viru A. Research methodology: endocrinologic measurements in exercise science and sports medicine. J Athl Training 2008; 43: 631-639
• 13 Hafner SM, Miettinen H, Karhapaa P, Mykkanen L, Laakso M. Leptin concentrations, sex hormones, and cortisol in nondiabetic men. J Clin Endocrinol Metab 1997; 82: 1807-1809
• 14 Harriss DJ, Atkinson G. Update – Ethical standards in sport and exercise science research. Int J Sports Med 2011; 32: 819-821
• 15 Horne L, Bell G, Fisher B, Warren S. Interaction between cortisol and tumour necrosis factor with concurrent resistance and endurance training. Clin J Sport Med 1997; 7: 247-251
• 16 Hulver MW, Houmard JA. Plasma leptin and exercise: recent findings. Sports Med 2003; 33: 473-482
• 17 Izquierdo M, Ibáñez J, Hakkinen K, Kraemer WJ, Ruesta M, Gorostiaga EM. Maximal strength and power, muscle mass, endurance and serum hormones in weightlifters and road cyclists. J Sports Sci 2004; 22: 465-478
• 18 Izquierdo M, Ibañez J, Calbet JAL, Navarro-Amezqueta I, González-Izal M, Idoate F. Cytokine and hormone responses to resistance training. Eur J Appl Physiol 2009; 107: 397-409
• 19 Jürimäe J, Jürimäe T. Leptin responses to short term exercise in college level male rowers. Br J Sports Med 2005; 39: 6-9
• 20 Kanaley JA, Fenicchia LM, Miller CS, Carhart R, Azevedo JL. Resting leptin responses to acute and chronic resistance training in type 2 diabetic men and women. Int J Obes Relat Metab Disord 2001; 25: 1474-1480
• 21 Keller P, Keller C, Steensberg A, Robinson LE, Pedersen BK. Leptin gene expression and systemic levels in healthy men: effect of exercise, carbohydrate, interleukin-6, and epinephrine. J Appl Physiol 2005; 98: 1805-1812
• 22 Kraemer WJ, Volek JS, Bush JA, Putukian M, Sebastianelli WJ. Hormonal responses to consecutive days of heavy-resistance exercise with or without nutritional supplementation. J Appl Physiol 1998; 85: 1544-1555
• 23 Landt M, Lawson GM, Helgeson JM, Ladenson JH, Jaffe AS, Hickner RC. Prolonged exercise decreases serum leptin concentrations. Metabolism 1997; 46: 1109-1112
• 24 Leveritt M, Abernethy PJ, Barry P, Logan PA. Concurrent strength and endurance training: the influence of dependent variable selection. J Strength Cond Res 2003; 17: 503-508
• 25 Licinio J, Mantzoros C, Negrão AB, Cizza G, Bongiorno PB, Wong M, Chrousos GP, Karp B, Allen C, Fler JS, Gold PW. Human leptin levels are pulsatile and inversely related to pituitary-adrenal function. Nat Med 1997; 3: 575-579
• 26 Licinio J. Longitudinally Sampled Human Plasma Leptin and Cortisol Concentrations Are Inversely Correlated. J Clin Endocrinol Metab [Letters to Editor] 1998; 33
• 27 Marfell-Jones M. International Standards for Anthropometric Assessment. ISAK: Potchefsroom, South Africa; 2006
• 28 Matsumoto AM, Bremner WJ. Modulation of pulsatile gonadotropin secretion by testosterone in man. J Clin Endocrinol Metab 1984; 58: 609-614
• 29 Negrão AB, Licinio J. Leptina: o Diálogo entre Adipócitos e Neurônios. Arq Bras Endocrinol Metab 2000; 44: 205-214
• 30 Nihiser AJ, Lee SM, Wechsler H, McKenna M, Odom E, Reinold C, Thompson D, Grummer-Strawn L. BMI Measurement in schools. Pediatrics 2009; 124: S89-S97
• 31 Pe’russe L, Collier G, Gagnon J, Leon AS. Acute and chronic effects of exercise on leptin levels in humans. J Appl Physiol 1997; 83: 5-10
• 32 Port K. Serum and saliva cortisol responses and blood lactate accumulation during incremental exercise testing. Int J Sports Med 1991; 12: 490-494
• 33 Robertson RJ, Goss FL, Dubé J, Rutkowski J, Dupain M, Brennan C, Andreacci J. Validation of the Adult OMINI Scale of Perceived Exertion for cycle ergometer exercise. Med Sci Sports Exerc 2004; 36: 102-108
• 34 Rosa G, Abdalla A, Dantas EHM, Mello DBd. Efeitos de distintas ordens de execução do treinamento concorrente sobre os níveis de cortisol de adultos fisicamente ativos. Revista Brasileira de Ciências da Saúde 2010; 8: 8-13
• 35 Rosa G, Cruz I, Mello DBd, Fortes MdSR, Dantas EHM. Plasma levels of leptin in overweight adults undergoing concurrent training. Int SportMed J 2010; 11: 356-362
• 36 Rosa G, Mello DBd, Biehl C, Dantas E. Níveis de cortisol em adultos com sobrepeso submetidos a treinamento concorrente. Braz J Sports Exerc Res 2010; 1: 11-15
• 37 Rosa G, Mello DBd, Daoud R, Cruz I, Dantas E. Concentración de Leptina en adultos con sobrepeso sujetos a un entrenamiento concurrente. Mot Hum 2010; 10: 95-102
• 38 Rose R, Kreuz L, Holaday J, Sulak K, Johnson C. Diurnal variation of plasma testosterone and cortisol. J Endocrinol 1972; 54: 177-178
• 39 Simsch C, Lormes W, Petersen K, Baur S, Liu Y, Hackney A, Lehmann M, Steinacker JM. Training intensity influences leptin and thyroid hormones in highly trained rowers. Int J Sports Med 2002; 23: 422-427
• 40 Sinha M. Ultradian oscillations of leptin secretion in humans. Biochem Biophys Res Commun 1996; 228: 733-738
• 41 Thomas JR, Nelson JK, Silverman SJ. Research Methods in Physical Activity. Human Kinetics Publishers; 2010
• 42 W.M.A. DECLARATION OF HELSINKI Ethical Principles for Medical Research Involving Human Subjects. 59^th WMA General Assembly, Seoul 2008