Insulin Resistance Induced by Zymosan as a New Animal Model in Mice

Abstract

Insulin resistance (IR) is known as a main problem in diabetic disorders. Some animal models for research in IR have been mentioned. Each model shows merit with some disadvantages. Thus, a new animal model for IR is required. The present study used zymosan, a mixture of cell-wall particles from the yeast named Saccharomyces cerevisiae, to establish a new model of IR in mice. Also, we compared the difference of this model with fructose-rich chow-induced model and found some merits of this model. Moreover, we identified that this model induced by zymosan is reversible and IR can be reversed gradually after termination of treatment. Taken together, we suggest zymosan as a useful agent to induce IR through inflammatory pathway in mice.

Key words

insulin resistance - zymosan - fructose-rich chow - western blot - mice

^# The first and second author contributed equally to the article.

• References

• 1 Gagliardi L, Wittert G. Management of obesity in patients with type 2 diabetes mellitus. Curr Diabetes Rev 2007; 3: 95-101
• 2 Kalofoutis C, Piperi C, Kalofoutis A, Harris F, Phoenix D, Singh J. Type II diabetes mellitus and cardiovascular risk factors: Current therapeutic approaches. Exp Clin Cardiol 2007; 12: 17-28
• 3 Soumaya K. Molecular mechanisms of insulin resistance in diabetes. Adv Exp Med Biol 2012; 771: 240-251
• 4 Saini V. Molecular mechanisms of insulin resistance in type 2 diabetes mellitus. World J Diabetes 2010; 1: 68-75
• 5 Kim S, Jwa H, Yanagawa Y, Park T. Extract from Dioscorea batatas ameliorates insulin resistance in mice fed a high-fat diet. J Med Food 2012; 15: 527-534
• 6 Ko WC, Liu IM, Chung HH, Cheng JT. Activation of I(2)-imidazoline receptors may ameliorate insulin resistance in fructose-rich chow-fed rats. Neurosci Lett 2008; 448: 90-93
• 7 Ionut V, Liu H, Mooradian V, Castro AV, Kabir M, Stefanovski D, Zheng D, Kirkman EL, Bergman RN. Novel canine models of obese prediabetes and mild type 2 diabetes. Am J Physiol Endocrinol Metab 2010; 298: E38-E48
• 8 Vicente AM, Guillen MI, Alcaraz MJ. Modulation of haem oxygenase-1 expression by nitric oxide and leukotrienes in zymosan-activated macrophages. Br J Pharmacol 2001; 133: 920-926
• 9 Speer CP, Ambruso DR, Grimsley J, Johnston Jr RB. Oxidative metabolism in cord blood monocytes and monocyte-derived macrophages. Infect Immun 1985; 50: 919-921
• 10 Xie K, Yu Y, Zhang Z, Liu W, Pei Y, Xiong L, Hou L, Wang G. Hydrogen gas improves survival rate and organ damage in zymosan-induced generalized inflammation model. Shock 2010; 34: 495-501
• 11 Volman TJ, Goris RJ, Lomme RM, DeGroot J, Verhofstad AA, Hendriks T. Increased expression of matrix metalloproteinases in the murine zymosan-induced multiple organ dysfunction syndrome. J Pathol 2004; 203: 968-975
• 12 Cuzzocrea S, Rossi A, Serraino I, Di Paola R, Dugo L, Genovese T, Britti D, Sciarra G, De Sarro A, Caputi AP, Sautebin L. Role of 5-lipoxygenase in the multiple organ failure induced by zymosan. Intensive Care Med 2004; 30: 1935-1943
• 13 Riu E, Ferre T, Hidalgo A, Mas A, Franckhauser S, Otaegui P, Bosch F. Overexpression of c-myc in the liver prevents obesity and insulin resistance. FASEB J 2003; 17: 1715-1717
• 14 Iochida LC, Tominaga M, Matsumoto M, Sekikawa A, Sasaki H. Insulin resistance in septic rats – a study by the euglycemic clamp technique. Life Sci 1989; 45: 1567-1573
• 15 Petit F, Bagby GJ, Lang CH. Tumor necrosis factor mediates zymosan-induced increase in glucose flux and insulin resistance. Am J Physiol 1995; 268: E219-E228
• 16 Davis JE, Gabler NK, Walker-Daniels J, Spurlock ME. The c-Jun N-terminal kinase mediates the induction of oxidative stress and insulin resistance by palmitate and toll-like receptor 2 and 4 ligands in 3T3-L1 adipocytes. Horm Metab Res 2009; 41: 523-530
• 17 Davis JE, Braucher DR, Walker-Daniels J, Spurlock ME. Absence of Tlr2 protects against high-fat diet-induced inflammation and results in greater insulin-stimulated glucose transport in cultured adipocytes. J Nutr Biochem 2011; 22: 136-141
• 18 Hsu JH, Wu YC, Liu IM, Cheng JT. Dioscorea as the principal herb of Die-Huang-Wan, a widely used herbal mixture in China, for improvement of insulin resistance in fructose-rich chow-fed rats. J Ethnopharmacol 2007; 112: 577-584
• 19 Szaroma W, Dziubek K. Influence of zymosan A on the content of ascorbic acid in mice. Chin J Physiol 2012; 55: 16-21
• 20 Lin JD, Chiou WK, Chang HY, Liu FH, Weng HF. Serum uric acid and leptin levels in metabolic syndrome: a quandary over the role of uric acid. Metabolism 2007; 56: 751-756
• 21 Kuo DH, Yeh CH, Shieh PC, Cheng KC, Chen FA, Cheng JT. Effect of shanzha, a Chinese herbal product, on obesity and dyslipidemia in hamsters receiving high-fat diet. J Ethnopharmacol 2009; 124: 544-550
• 22 Chiu YJ, Chung HH, Yeh CH, Cheng JT, Lo SH. Improvement of Insulin Resistance by Chlorella in Fructose-rich Chow-fed Rats. Phytother Res 2011; 25: 1306-1312
• 23 Su CH, Liu IM, Chung HH, Cheng JT. Activation of I2-imidazoline receptors by agmatine improved insulin sensitivity through two mechanisms in type-2 diabetic rats. Neurosci Lett 2009; 457: 125-128
• 24 Moreira AP, Cavassani KA, Ismailoglu UB, Hullinger R, Dunleavy MP, Knight DA, Kunkel SL, Uematsu S, Akira S, Hogaboam CM. The protective role of TLR6 in a mouse model of asthma is mediated by IL-23 and IL-17A. J Clin Invest 2011; 121: 4420-4432
• 25 Hou L, Xie K, Li N, Qin M, Lu Y, Ma S, Ji G, Xiong L. 100% oxygen inhalation protects against zymosan-induced sterile sepsis in mice: the roles of inflammatory cytokines and antioxidant enzymes. Shock 2009; 32: 451-461
• 26 Carstensen M, Herder C, Kempf K, Erlund I, Martin S, Koenig W, Sundvall J, Bidel S, Kuha S, Roden M, Tuomilehto J. Sfrp5 correlates with insulin resistance and oxidative stress. Eur J Clin Invest 2013; 43: 350-357
• 27 Piyathilake CJ, Badiga S, Alvarez RD, Partridge EE, Johanning GL. A Lower Degree of PBMC L1 Methylation is associated with excess body weight and higher HOMA-IR in the presence of lower concentrations of plasma folate. Plos One 2013; 8: e54544
• 28 Lalli CA, Pauli JR, Prada PO, Cintra DE, Ropelle ER, Velloso LA, Saad MJ. Statin modulates insulin signaling and insulin resistance in liver and muscle of rats fed a high-fat diet. Metabolism 2008; 57: 57-65
• 29 Bandyopadhyay GK, Yu JG, Ofrecio J, Olefsky JM. Increased p85/55/50 expression and decreased phosphotidylinositol 3-kinase activity in insulin-resistant human skeletal muscle. Diabetes 2005; 54: 2351-2359
• 30 Tanti JF, Gual P, Grémeaux T, Gonzalez T, Barrès R, Le Marchand-Brustel Y. Alteration in insulin action: role of IRS-1 serine phosphorylation in the retroregulation of insulin signalling. Ann Endocrinol 2004; 65: 43-48
• 31 Perseghin G, Petersen K, Shulman GI. Cellular mechanism of insulin resistance: potential links with inflammation. Int J Obes Relat Metab Disord 2003; 27 (Suppl. 03) S6-S11
• 32 Hokayem M, Blond E, Vidal H, Lambert K, Meugnier E, Feillet-Coudray C, Coudray C, Pesenti S, Luyton C, Lambert-Porcheron S, Sauvinet V, Fedou C, Brun JF, Rieusset J, Bisbal C, Sultan A, Mercier J, Goudable J, Dupuy AM, Cristol JP, Laville M, Avignon A. Grape Polyphenols Prevent Fructose-Induced Oxidative Stress and Insulin Resistance in First-Degree Relatives of Type 2 Diabetic Patients. Diabetes care 2012; [Epub ahead of print]
• 33 Qin B, Anderson RA. An extract of chokeberry attenuates weight gain and modulates insulin, adipogenic and inflammatory signalling pathways in epididymal adipose tissue of rats fed a fructose-rich diet. Br J Nutr 2012; 108: 581-587
• 34 Hashikawa-Hobara N, Hashikawa N, Inoue Y, Sanda H, Zamami Y, Takatori S, Kawasaki H. Candesartan cilexetil improves angiotensin II type 2 receptor-mediated neurite outgrowth via the PI3K-Akt pathway in fructose-induced insulin-resistant rats. Diabetes 2012; 61: 925-932
• 35 Madani Z, Louchami K, Sener A, Malaisse WJ, Ait Yahia D. Dietary sardine protein lowers insulin resistance, leptin and TNF-alpha and beneficially affects adipose tissue oxidative stress in rats with fructose-induced metabolic syndrome. Int J Mol Med 2012; 29: 311-318
• 36 Wu HT, Chang CK, Tsao CW, Wen YJ, Ling SM, Cheng KC, Chang CJ, Cheng JT. Insulin resistance without obesity induced by cotton pellet granuloma in mice. Lab Invest 2009; 89: 362-369