Synthesizing Clickable Glutathione by Glutathione Synthetase Mutant for Detecting Protein Glutathionylation

Kusal T. G. Samarasinghe; Young-Hoon Ahn

doi:10.1055/s-0034-1379946

Synlett, Inhaltsverzeichnis

Synlett 2015; 26(03): 285-293
DOI: 10.1055/s-0034-1379946

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Synthesizing Clickable Glutathione by Glutathione Synthetase Mutant for Detecting Protein Glutathionylation

Kusal T. G. Samarasinghe

Wayne State University, Chemistry Building, Cass Avenue, Detroit, MI 48202, USA eMail: yahn@chem.wayne.edu

,

Young-Hoon Ahn*

Wayne State University, Chemistry Building, Cass Avenue, Detroit, MI 48202, USA eMail: yahn@chem.wayne.edu

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Abstract

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Abstract

In response to reactive oxygen species (ROS), glutathione plays an important role in redox signaling by forming a disulfide bond with protein cysteine residues, known as glutathionylation. We briefly review the roles of glutathione, ROS, and glutathionylation in redox regulation. We then introduce common biochemical methods for identifying glutathionylation and highlight our recent chemical method for selective detection of glutathionylation. The merits, limitations, and future applications of our approach are also discussed.

Key words

protein cysteine modification - glutathionylation - enzyme engineering - reactive oxygen species - redox signaling

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Referenzen

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