Contact Activation of Hageman Factor and the Interaction of Hageman Factor and Plasma Thromboplastin Antecedent

Summary

Experiments with highly purified activated bovine Hageman factor revealed that surface activation of Hageman factor is effective only when PTA is present simultaneously. Surface activation of Hageman factor probably is not caused only by an alteration of the Hageman factor itself but by the catalyzing action of surface on the reaction between this factor and PTA, possibly by preferential adsorption of these factors. Arguments are given for the supposition that Hageman factor forms a complex with PTA which is unstable and probably identical with the so-called activation product. The instability of this activation product is caused by deterioration of PTA while Hageman factor remains. The process of contact activation stops because further Hageman factor activity is blocked by PTA which sticks to Hageman factor even after it is deteriorated. In normal circumstances not all Hageman factor molecules are adsorbed to a surface by the competing effect of other proteins in the plasma with glass affinity. Exposing plasma to a large surface such as provided by celite, causes complete adsorption of all Hageman factor molecules and in this way causes complete consumption of PTA.

^* This study was supported in part by the Organization for Health Research, T N O, The Netherlands.

• References

• 1 Ratnoff O. D, Rosenblum J. M. Role of Hageman factor in the initiation of clotting by glass : Evidence that glass frees Hageman factor from inhibition. Amer. J. Med 25: 160 1958;
• 2 Biggs R, Sharp A. A, Margolis J, Hardisty R. M, Stewart J, Davidson W. M. Defects in the early stages of blood coagulation : A report of four cases. Brit. J. Haemat 4: 111 1958;
• 3 Soulier J. P, Wartelle O, Ménaché D. Hageman trait and P. T. A. deficiency: The role of contact of blood with glass. Brit. J. Haemat 5: 121 1959;
• 4 Ratnoff O. D, Davie E. W, Mallet D. L. Studies on the action of Hageman factor: Evidence that activated Hageman factor in turn activates plasma thromboplastin antecedent. J. clin. Invest 40: 803 1961;
• 5 Ratnoff O. D, Davie E. W. The activation of Christmas factor (Factor IX) by activated plasma thromboplastin antecedent (activated Factor XI). Biochemistry 1: 677 1962;
• 6 Ratnoff O. D, Davie E. W. The purification of activated Hageman factor (activated Factor XII). Biochemistry 1: 967 1962;
• 7 Nossel H. L. The Contact Phase of Blood Coagulation. Blackwell Scientific Publications; Oxford: 1964
• 8 Schoenmakers J. G. G, Matze R, Haanen G, Zilliken F. Hageman factor, a novel Sialoglyco-protein with esterase activity. Biochim. biophys. Acta (Amst.) 101: 166 1965;
• 9 Haanen C, Hommes F, Benraad H, Morselt G. A case of Hageman factor deficiency and a method to purify the factor. Thrombos. Diathes. haemorrh. (Stuttg.) 5: 201 1960;
• 10 Haanen C, Schoenmakers J. G. G. One-stage assay for Hageman factor (Factor XII). Further studies on the role of Hageman factor in blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 557 1963;
• 11 Horowitz H. I, Wilcox W. P, Fujimoto M. M. The use of artificially depleted normal plasma for the assay of Factor XI (P.T. A.). Fed. Proc 22: 162 1963;
• 12 Schoenmakers J. G. G, Kurstjens R. M, Haanen G, Zilliken F. Purification of activated bovine Hageman factor. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 546 1963;
• 13 Hardisty R. M, Margolis J. The role of Hageman factor in the initiation of blood coagulation. Brit. J. Haemat 5: 203 1959;
• 14 Ratnoff O. D, Grum J. D. Activation of Hageman factor by solutions of ellagic acid. J. Lab. clin. Med 63: 359 1964;
• 15 Schoenmakers J, Matze R, Haanen G, Zilliken F. Proteolytic activity of purified bovine Hageman factor. Biochim. biophys. Acta (Amst.) 93: 433 1964;
• 16 Schoenmakers J. G. G. Purification and properties of Hageman factor. Thesis, University of Nijmegen, 1965.
• 17 Speer R. J, Ridgway H, Hill J. M. Activated human Hageman factor (XII). Thrombos. Diathes. haemorrh. (Stuttg.) 14: 1 1965;
• 18 Didisheim P. Panel Discussion. Xth Congress of the International Society of Haematology, Stockholm 1964.
• 19 Margolis J. Initiation of blood coagulation by glass and related surfaces. J. Physiol 137: 95 1957;
• 20 Ratnoff O. D. An enzyme in plasma inactivating Hageman factor. J. clin. Invest 37: 923 1958;
• 21 Haanen G, Hommes F, Morselt G. Some observations on the role of Hageman factor in blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 6: 261 1961;
• 22 Vroman L. Behavior of coagulation factors at interfaces. Biophysical mechanism in vascular homeostasis and intravascular thrombosis 81 Ed. Sawyer P. N. Appleton-Century-Crofts; New York: 1965
• 23 Soulier J. P, Prou-Wartelle O. New data on Hageman factor and plasma thromboplastin antecedent: The role of “contact” in the initial phase of blood coagulation. Brit. J. Haemat 6: 88 1960;
• 24 Waaler B. A. Contact activation in the intrinsic blood clotting system. Scand. J. clin. Lab. Invest. 11 37 1959
• 25 Nossel H. L, Niemetz J. The contact product inhibitor. Clin. Res 12: 228 1964;
• 26 Biggs R, Macfarlane R. G, Denson K. W. E, Ash B. J. Thrombin and the interaction of factors VIII and IX. Brit. J. Haemat 11: 276 1965;
• 27 Hanahan D. J, Papahadjopoulos D. Interactions of phospholipids with coagulation factors. Genetics and the interaction of blood clotting factors. 71 Ed. Koller F. Schattauer; Stuttgart: 1965
• 28 Alkjaersig N, Fletcher A. P, Sherry S. The Mechanism of clot dissolution by plasmin. J. clin. Invest 38: 1086 1959;
• 29 Dudok de Wit G, Krijnen H. W, den Ottolander G. J. H. The measurement of fibrinolytic activity with ¹³¹J-labeled clots. I. Methods. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 315 1962;