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Thromb Haemost 1967; 17(03/04): 381-387
DOI: 10.1055/s-0038-1654161
Originalarbeiten - Original Articles - Travaux Originaux
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Effects of Thrombin on Factor VIII Activity: Stabilization of Factor VIII by Glycine Ethyl Ester

C. T Kisker   *
1   U. S. Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, Division of Biologies Standards, Bethesda, Maryland
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Publication Date:
26 June 2018 (online)

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Summary

The action of purified thrombin on a preparation of antihemophilic factor with fibrinogen has been studied. In the absence of active thrombin (thrombin inactivated with DFP) polymerization of fibrin by calcium was accompanied by significant losses of AHF. In the presence of thrombin and calcium, 0.3 M glycine ethyl ester resulted in a significant reduction in the loss of AHF activity.

* Present Address: Department of Pediatrics, Cincinnati Childrens Hospital, Cincinnati, Ohio.


 

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