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Synfacts 2020; 16(05): 0601
DOI: 10.1055/s-0040-1707485
DOI: 10.1055/s-0040-1707485
Chemistry in Medicine and Biology
Synthesis of Photo-Caged ATP
Further Information
Publication History
Publication Date:
20 April 2020 (online)
Significance
The release of inorganic phosphate from ATP is crucial to numerous life processes from cell movement to cell signaling. In 1978, Kaplan et al. expanded the concept of photolabile protecting groups to ATP and demonstrated the controlled release of ATP to activate Na+/K+ pumps in resealed red blood cell ghosts.
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Comment
The photolabile ‘caged phosphate’ was coupled to ADP through a DCC mediated phosphorylation. Upon irradiation with UV light, the caged ATP is photolyzed, releasing ATP and a reactive nitrosoketone. The ATPase inhibition of the Na+/K+ pump caused by the nitrosoketone by-product can be reversed by incorporating glutathione into the assay.
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