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Planta Med 2001; 67(2): 168-171
DOI: 10.1055/s-2001-11497
Letter

© Georg Thieme Verlag Stuttgart · New York

Essential Oils and Hexane Extracts from Leaves and Fruits of Cistus monspeliensis. Cytotoxic Activity of ent-13-epi-Manoyl Oxide and its Isomers

Dimitra Angelopoulou1 , Costas Demetzos1,*, Costas Dimas2 , Dimitrios Perdetzoglou1 , Anargyros Loukis1
  • 1 School of Pharmacy, Department of Pharmacognosy, University of Athens, Greece
  • 2 International Institute of Anticancer Research, Attiki, Greece
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Publication History

March 7, 2000

May 31, 2000

Publication Date:
31 December 2001 (online)

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Abstract

Essential oils and hexane extracts from Cistus monspeliensis L. leaves and fruits were analysed by GC-MS. Manoyl oxide and its isomers, 3β-hydroxy-manoyl oxide, 3β-hydroxy-13-epi-manoyl oxide, as well as 3β-acetoxy-13-epi-manoyl oxide were detected for the first time in C. monspeliensis L. Ent-13-epi-manoyl oxide was isolated from the hexane extract of leaves and its structure was determined using spectroscopic methods. In vitro cytotoxic activity of ent-13-epi-manoyl oxide and mixtures of manoyl oxide isomers at 10-4 M concentrations ranged from 11.1 to 32.2 % of the activity for a vinblastine control evaluated against nine leukemic cell lines. The results showed that the manoyl oxide isomer mixtures as well as ent-13-epi-manoyl oxide exhibited a slight growth inhibiting activity.

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Ass. Prof. Dr. Costas Demetzos

School of Pharmacy

Department of Pharmacognosy

Panepistimiopolis Zografou 15771

Athens

Greece

Email: demetzos@galenos.pharm.uoa.gr

Fax: +30-1-7274596