References
1
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Murakami T.
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Tanabe G.
Muraoka O.
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Murakami T.
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Morikawa T.
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Johnston BD.
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10a
Reichardt C.
Solvents
and Solvent Effects in Organic Chemistry
VCH;
Weinheim:
1996.
2nd
Ed..
p.137-147
10b
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Solvents and Solvent Effects in Organic Chemistry
VCH;
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p.359-384
11 Snider, B. B.; Lobera, M. unpublished
results.
12
2,3,5-Tri-
O
-benzyl-1,4-dideoxy-1,4-{[(2S,3S)-2,4-di-(benzyloxy)-3-sulfooxy)butyl]-
episulfoniumylidene}-
d
-arabinitol Inner
Salt (
11).
A mixture of the
thioether 2
[5]
(270
mg, 0.64 mmol)and 2,4-di-O-benzyl-1,3-cyclic
sulfate(10)
[6]
[9]
(280 mg, 0.77 mmol) in either acetone
or HFIP (0.5 mL), containing anhydrous K2CO3 (16
mg, 0.10 mmol) was stirred in a sealed tube in an oil-bath (75-80 °C)
for 14h. The solvent was removed under reduced pressure and the
residue was purified by column chromatography using (CH2Cl2-MeOH,
10:1) as eluant to give the title compound 11, as an amorphous solid (29 mg, 5%)
in acetone and (229 mg, 45%) in HFIP. Rf 0.40 (CH2Cl2-MeOH,
10:1); [α]D - 26
(c 1.3, CHCl3); 1H
NMR (CDCl3): δ = 7.38-7.05
(25 H, m, Ar), 4.67 and 4.45 (2 H, 2d, J
A,B = 11.8
Hz, CH
2Ph), 4.60 and 4.45
(2 H, 2d, J
A,B = 9.5
Hz, CH
2Ph), 4.59 and 4.44
(2 H, 2d, J
A,B = 11.2 Hz,
CH
2Ph), 4.58 (1 H, dt, J
2
′
,3
′ = 5.0
Hz, H-3′), 4.42 and 4.28 (2 H, 2d, J
A,B = 11.0
Hz, CH
2Ph), 4.36 (1 H, m,
H-2), 4.32 (1 H, ddd, J = 1.7,
4.1, 6.3 Hz, H-2′), 4.30 and 4.20 (2 H, 2d, J
A,B = 11.7
Hz, CH
2Ph), 4.23 (1 H, m,
H-3), 4.13 (1 H, dd, J
1
′
a,1
′
b = 13.4, J
1
′
a,2
′ = 2.0
Hz, H-1′a), 4.05 (1 H, d, J
2,3 = 13.3
Hz, H-1a), 4.00 (1 H, dd, J
4
′
a,4
′
b = 11.1,
J3
′
,4
′
a = 2.7 Hz,
H-4′a), 3.86 (1 H, dd, J
3
′
,4
′
b = 2.4, J
4
′
a,4
′
b = 11.3
Hz, H-4′b), 3.71 (1 H, br t, J = 9.2
Hz, H-4), 3.69 (1 H, dd, J
1
′
b,2
′ = 3.8, J
1
′
b,1
′
a = 9.2
Hz, H-1′b), 3.60 (1 H, dd, J
1a,1b = 13.5,
J1b,2 = 3.8 Hz, H-1b), 3.51
(1 H, dd, J
5a,5b = 13.6,
J4,5a = 9.7 Hz, H-5a), 3.49
(1 H, dd, J
4,5b = 9.7 Hz,
H-5b); 13C NMR (CDCl3): δ = 137.97,
136.77, 136.71, 136.05 and 135.77 (5 ¥ Cipso Ph),
128.81-127.66 (25 C, Ph), 83.14 (C-3), 81.65 (C-2), 74.59
(C-3′), 73.81, 73.53, 3.39, 72.12, 71.84 (5 ¥ CH2Ph), 73.10 (C-2′),
68.79 (C-4′), 66.62 (C-5), 65.53 (C-4), 50.89 (C-1′),
48.07 (C-1). MALDI-TOF MS: m/z 785.41 (M+ + H),
808.32 (M+ + Na). Anal. Calcd for C44H48O9S2:
C, 67.32; H, 6.16. Found: C, 67.36; H, 6.10.
13
2,3,5-Tri-
O
-benzyl-1,4-dideoxy-1,4-{[(2S,3S)-2,4
-benzylidenedioxy-3-(sulfooxy)butyl]-episulfonium-ylidene}-
d
-arabinitol
Inner Salt (
4).
A mixture
of the thioether 2
[5]
(260
mg, 0.62 mmol)and 2,4-di-O-benzylidene-1,3-cyclic
sulfate(3)
[5]
(200
mg, 0.74 mmol) in either acetone or HFIP (0.5 mL) containing K2CO3 (13
mg, 0.09 mmol) was treated as described above to yield the title compound 4
[5]
as an amorphous solid (252 mg,
59% in acetone) and (406 mg, 94% in HFIP).
14
1,4-Anhydro-2,3,5-tri-
O
-( p
-methoxybenzyl)-4-thio-
d
-arabinitol
(
12).
To an ice-cold mixture
of 1,4-anhydro-4-thio-d-arabinitol 7
[5]
(0.98
g, 6.52 mmol)and 60% NaH (1.56 g, 39.15 mmol, 6 equiv)
in THF (15 mL), a solution of p-methoxybenzyl chloride
(4.59 g, 29.34 mmol, 4.5 equiv) in THF (10 mL) was added over 30
min. The reaction mixture was allowed to attain room temperature
and further stirred for 1 h before heating to 55 °C for
12 h. The reaction mixture was cooled and poured in to ice-water
(150 mL) and extracted with Et2O (150 mL). The organic
solution was dried (Na2SO4) and concentrated.
The product was purified by column chromatography [hexanes-EtOAc,
7:3] to give a colorless syrup (2.96 g, 87%). [α]D +6
(c 1, CHCl3); 1H
NMR (CDCl3): δ = 7.20-6.80
(12 H, m, Ar), 4.55 (2 H, s, CH
2Ph), 4.48
and 4.45 (2 H, 2d, J
A,B = 11.7
Hz, CH
2Ph), 4.42 and 4.39
(2 H, 2d, J
A,B = 12.0
Hz, CH
2Ph), 4.13 (1 H, dd, J
1a,2 = 4.6, J
2,3 = 9.1
Hz, H-2), 4.05 (1 H, dd, J
2,3 = J
3,4 = 3.7 Hz,
H-3), 3.81 (3 H, s, OCH
3),
3.79 (3 H, s, OCH
3), 3.76
(3 H, s, OCH
3), 3.64 (1 H,
dd, J
5a,5b = 8.9, J
4,5a = 7.5
Hz, H-5a), 3.50 (1 H, ddd, J
4,5b = 6.3
Hz, H-4), 3.45 (1 H, dd, H-5b), 3.04 (1 H, dd, J
1a,1b = 11.4, J
1a,2 = 5.2
Hz, H-1a), 2.85 (1 H, dd, H-1b). 13C
NMR (CDCl3): δ = 159.24, 159.16 (3 ¥ Cpara), 130.31,
130.19, 130.01 (3 Cipso), 129.48, 129.28, 129.22 (6 ¥ Cortho),
113.80, 113.74 (6 ¥ Cmeta), 84.77 (C-3), 84.70
(C-2), 72.66, 71.49, 71.20 (3 ¥ CH2Ph), 72.15 (C-5), 55.24 (3 ¥ OCH3), 48.96 (C-4), 33.07 (C-1).
Anal. Calcd for C29H34O6S: C, 68.21;
H, 6.71. Found: C, 67.99; H, 6.69.
15
2,3,5-Tri-
O
-
p
-Methoxybenzyl-1,4-dideoxy-1,4-{[(2
S
,3
S
)-2,4-benzylidenedioxy-3-(sulfooxy)butyl]-episulfonium-ylidene}-
d
-arabinitol
Inner Salt (
13). A mixture of the thioether 12 (1.50 g, 2.94 mmol), and the cyclic
sulfate 3 (0.96 g, 1.2 equiv) in HFIP (2.5
mL) containing anhydrous K2CO3 (30 mg) was
stirred in a sealed tube in an oil-bath (55°C) overnight.
TLC analysis (CH2Cl2-MeOH, 10:1) showed
that the thioether 12 was completely consumed.
The solvent was removed under reduced pressure and the product was
purified by column chromatography (gradient of CH2Cl2 to
CH2Cl2-MeOH, 10:1) to give compound 13 (2.3 g, 100%) as a colorless
foam. [α]D -10.5
(c 1.1, CH2Cl2); 1H NMR
(CD2Cl2): δ = 7.51-6.81
(17 H, m, Ph), 5.53 (1 H, s, C6H5CH), 4.57 (1 H, ddd, J
2
′
,3
′ = J
3
′
,4
′
ax = 10.0, J
3
′
,4
′
eq = 5.5 Hz,
H-3′), 4.49 (1 H, dd, J
4
′
ax,4
′
eq = 10.8
Hz, H-4′eq), 4.44 (2 H, s, CH
2Ph),
4.42-4.39 (1 H, m, H-2), 4.39 and 4.29 (2 H, 2d, J
A,B = 11.4
Hz, CH
2Ph), 4.33 (1 H, dd, J
1
′
a,1
′
b = 13.4, J
1
′
a,2
′ = 2.6
Hz, H-1′a), 4.29-4.26 (1 H, m, H-3), 4.26 (1 H, ddd,
H-2′), 4.19 and 4.09 (2 H, 2d, J
A,B = 11.5
Hz, CH
2Ph), 4.03 (1 H, br
d, J
1a,2 <1 Hz, H-1a),
3.96-3.89 (2 H, m, H-4, H-1′b), 3.80 (3 H, s,
OCH3), 3.79 (3 H, s, OCH3), 3.78 (3 H, s,
OCH3), 3.77 (1 H, dd, H-4′ax), 3.63 (1 H, dd, J
1a,1b = 13.3, J
1b,2 = 3.8
Hz, H-1b), 3.58 (1 H, dd, J
5a,5b = 9.9, J
4,5a = 8.5 Hz,
H-5a), 3.49 (1 H, dd, J
4,5b = 7.3
Hz, H-5b); 13C NMR (CD2Cl2): δ 160.30,
160.23, 159.97, 137.20 and 130.27-126.61 (21 ¥ C,
Ph), 114.45, 114.36 and 114.18 (3 ¥ C
ipso
, OMBn), 101.96 (PhCH), 83.29 (C-3), 82.37 (C-2), 76.76
(C-2′), 73.36, 72.43, and 72.14 (3 ¥ CH2Ph), 69.50 (C-4′),
66.71 (C-5), 66.55 (C-4), 66.45 (C-3′), 55.61 (3 C, 3 ¥ OCH3), 49.55 (C-1′),
48.48 (C-1). Anal. Calcd for C40H46O12S2:
C, 61.36; H, 5.92. Found: C, 61.13; H, 6.00.
16
1,4-Dideoxy-1,4-{[(2
S
,3
S
)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene}-
d
-arabinitol Inner
Salt(1). Compound 13 (2.30
g, 2.94 mmol) was dissolved in trifluoroacetic acid (24 mL) and
while stirring, water (2.4 mL) was added. The mixture was stirred
at room temperature for 0.5 h. The solvent was removed under reduced
pressure and the gummy residue was washed with CH2Cl2 (3 ¥ 20
mL). Water (15 mL) was added to dissolve the crude product, and
then evaporated under reduced pressure to remove the traces of remaining
acid. Salacinol (1) (0.67 g, 68%)
was crystallized from MeOH. The mother liquor was concentrated and
purified by column chromatography (EtOAc-MeOH-H2O,
7:3:1) to give more salacinol 1 as a white
solid (0.18 g, 18%).