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Planta Med 2004; 70(3): 284-287
DOI: 10.1055/s-2004-818927
Letter
© Georg Thieme Verlag Stuttgart · New York

Determination of Six Major Flavonoid Glycosides in Saussurea mongolica by Capillary Electrophoresis

Ting-Fu Jiang1 , Ya Li1 , Yan-Ping Shi1 , 2
  • 1Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, P. R. China
  • 2National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, P. R. China
This work was supported by the ”Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000, National Natural Science Foundation of China (NSFC 20021001), and Natural Science Foundation of Gansu Province (No. ZS021-A25-011-Z)
Further Information

Publication History

Received: August 14, 2003

Accepted: November 22, 2003

Publication Date:
23 March 2004 (online)

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Abstract

A new capillary electrophoresis (CE) method was established for simultaneous assay of six flavonoid O-glycosides, asebotin (AS), kaempferol 3-O-β-D-glucopyranoside (KG), kaempferol 3-O-α-L-rhamnopyranoside (KR), 7-methoxykaempferol 3-O-α-L-rhamnopyranoside (KMR), quercetin 3-O-β-D-glucopyranoside (QG) and quercetin 3-O-α-L-rhamnopyranoside (QR) obtained from Chinese herbal plant extract of Saussurea mongolica. The optimum buffer system was 30 mmol/L borate buffer (Na2B4O7/HCl, pH 9.00) with 40 % (v/v) methanol. Voltage was 15 kV and detection at 270 nm. The application of this method for the separation and determination of the six flavonoid O-glycosides in S. mongolica is reported. The relative standard deviations of migration times and peak areas were < 2.61 and 4.41 %, respectively. The contents of six natural products ranged from 0.043 to 0.60 mg/g and recoveries ranged from 92.4 to 104.4 %. The effects of pH value, buffer concentration, surfactant, β-cyclodextrin and organic modifier on the separation were investigated.

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Prof. Yan-Ping Shi

Lanzhou Institute of Chemical Physics

Chinese Academy of Sciences

Lanzhou 730000

Peoples Republic of China

Fax: +86-931-8277591

Email: shiyp@lzu.edu.cn

Email: shiyp @ns.lzb.ac.cn