Differential Expression of Three Purple Acid Phosphatases from Potato

P. Zimmermann; B. Regierer; J. Kossmann; E. Frossard; N. Amrhein; M. Bucher

doi:10.1055/s-2004-821091

Plant Biology, Inhaltsverzeichnis

Plant Biol (Stuttg) 2004; 6(5): 519-528
DOI: 10.1055/s-2004-821091

Original Paper

Georg Thieme Verlag Stuttgart KG · New York

Differential Expression of Three Purple Acid Phosphatases from Potato

P. Zimmermann¹ , B. Regierer² , J. Kossmann³ , E. Frossard¹ , N. Amrhein⁴ , M. Bucher¹

¹Swiss Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon, 8315 Lindau, Switzerland
²University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24 - 25, Haus 20, 14476 Golm, Germany
³Risø National Laboratory, Plant Research Department, 4000 Roskilde, Denmark
⁴Swiss Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Universitätsstrasse 2, 8092 Zurich, Switzerland

Abstract

Three cDNAs encoding purple acid phosphatase (PAP) were cloned from potato (Solanum tuberosum L. cv. Désirée) and expression of the corresponding genes was characterised. StPAP1 encodes a low-molecular weight PAP clustering with mammalian, cyanobacterial, and other plant PAPs. It was highly expressed in stem and root and its expression did not change in response to phosphorus (P) deprivation. StPAP2 and StPAP3 code for high-molecular weight PAPs typical for plants. Corresponding gene expression was shown to be responsive to the level of P supply, with transcripts of StPAP2 and StPAP3 being most abundant in P-deprived roots or both stem and roots, respectively. Root colonisation by arbuscular mycorrhizal fungi had no effect on the expression of any of the three PAP genes. StPAP1 mRNA is easily detectable along the root axis, including root hairs, but is barely detectable in root tips. In contrast, both StPAP2 and StPAP3 transcripts are abundant along the root axis, but absent in root hairs, and are most abundant in the root tip. All three PAPs described contain a predicted N-terminal secretion signal and could play a role in extracellular P scavenging, P mobilisation from the rhizosphere, or cell wall regeneration.

Key words

Root - root hairs - purple acid phosphatase - exudation - mycorrhiza.

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M. Bucher

Swiss Federal Institute of Technology (ETH) Zurich
Institute of Plant Sciences
Experimental Station Eschikon

8315 Lindau

Switzerland

eMail: marcel.bucher@ipw.biol.ethz.ch

Section Editor: J. Whelan