Origin and Evolution of the Light-Dependent Protochlorophyllide Oxidoreductase (LPOR) Genes

J. Yang; Q. Cheng

doi:10.1055/s-2004-821270

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2004; 6(5): 537-544
DOI: 10.1055/s-2004-821270

Original Paper

Georg Thieme Verlag Stuttgart KG · New York

Origin and Evolution of the Light-Dependent Protochlorophyllide Oxidoreductase (LPOR) Genes

J. Yang¹ , Q. Cheng²

¹College of Life Sciences, Peking University, Beijing 100871, China
²Department of Plant Sciences, University of Cambridge, Downing Site, Cambridge CB2 3EA, UK

Abstract

Light-dependent NADPH-protochlorophyllide oxidoreductase (LPOR) is a nuclear-encoded chloroplast protein in green algae and higher plants which catalyzes the light-dependent reduction of protochlorophyllide to chlorophyllide. Light-dependent chlorophyll biosynthesis occurs in all oxygenic photosynthetic organisms. With the exception of angiosperms, this pathway coexists with a separate light-independent chlorophyll biosynthetic pathway, which is catalyzed by light-independent protochlorophyllide reductase (DPOR) in the dark. In contrast, the light-dependent function of chlorophyll biosynthesis is absent from anoxygenic photosynthetic bacteria. Consequently, the question is whether cyanobacteria are the ancestors of all organisms that conduct light-dependent chlorophyll biosynthesis. If so, how did photosynthetic eukaryotes acquire the homologous genes of LPOR in their nuclear genomes? The large number of complete genome sequences now available allow us to detect the evolutionary history of LPOR genes by conducting a genome-wide sequence comparison and phylogenetic analysis. Here, we show the results of a detailed phylogenetic analysis of LPOR and other functionally related enzymes in the short chain dehydrogenase/reductase (SDR) family. We propose that the LPOR gene originated in the cyanobacterial genome before the divergence of eukaryotic photosynthetic organisms. We postulated that the photosynthetic eukaryotes obtained their LPOR homologues through endosymbiotic gene transfer.

Key words

Protochlorophyllide reductase - cyanobacterial origin - endosymbiotic gene transfer.

Full Text

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Q. Cheng

Department of Plant Sciences
University of Cambridge

Downing Site

Cambridge CB2 3EA

UK

Email: qc205@cam.ac.uk

Section Editor: W. Martin