Abstract
We have previously demonstrated that triiodothyronine (T3 ) stimulates hepatic IGFBP-4 expression in rats. Since there is evidence that some of the genes whose expression is regulated by T3 are also sensitive to 3,5-diiodothyronine (T2 ), we used the adult rat hepatocyte model in primary cultures directly exposed to T2 to evaluate insulin-like growth factor binding protein-4 (IGFBP-4) expression by Northern and Ligand blot analyses in this study. Our results demonstrate that T2 , like T3 , is able to enhance IGFBP-4 mRNA and protein after 12 - 24 h of incubation. The potency of the two iodothyronines is comparable as judged by dose-dependence experiments. The T2 -induced IGFBP-4 increase is independent from ongoing protein synthesis but dependent on active transcription. Since T3 and T2 do not affect IGF-I production, it appears that the iodothyronines affect the hepatic IGF system at the IGFBP level. Our data, demonstrating that T2 mimics the stimulatory effect of T3 on IGFBP-4 expression by rat hepatocytes, allow us to include IGFBP-4 gene among the genes regulated by the two iodothyronines.
Key words
Thyroid hormone - IGF - Liver
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Ilaria Demori
Dipartimento di Biologia Sperimentale · Ambientale ed Applicata (DIBISAA) · Sezione di Fisiologia Generale e Comparata · Università degli Studi di Genova
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