Multi-Spezies-Probiotika kombinieren die spezifischen Eigenschaften von verschiedenen Bakterien-Gattungen, -Arten oder -Stämmen miteinander und können im Idealfall synergistisch wirken. Auf (immuno)pathophysiologischer Grundlage wird das Design krankheitsspezifischer Multi-Spezies-Probiotika zur Vorbeugung vor Allergien im Kindesalter und Asthma beschrieben. Ein nach diesen Kriterien konzipiertes Handelspräparat aus Bifidobacterium bifidum, B. infantis und Lactobacillus lactis führte nach perinataler Verabreichung zu einer erheblichen Verringerung von Ekzemen in den ersten 3 Monaten nach der Geburt. Die Wirkung hielt auch darüber hinaus an. Es bleibt offen, ob dieses Probiotikum auch eine erfolgreiche Reduzierung von Asthma-Anfällen herbeiführen kann.
Summary
A literature survey was carried out to compare functionality and efficacy of monostrain, multistrain and multispecies probiotics. Multispecies probiotics offer the opportunity to combine genus, species or strain-specific properties and may provide synergistic effects. Through a combination of strains with distinct health-promoting properties, diseasespecific multispecies probiotics can be designed that tackle various pathophysiological aspects simultaneously. The design of a disease-specific multispecies probiotic for prevention of childhood allergy and asthma is described based on the (immuno)pathophysiological events in asthma and allergy. We have selected a commercially available multispecies probiotic consisting of Bifidobacterium bifidum, B. infantis and Lactobacillus lactis). Perinatal administration of this probiotic mixture significantly reduced the amount of eczema during the first 3 months of life when compared to placebo. The protective effects are sustained during the first years but it remains to be seen whether this protocol is able to reduce the incidence of asthma.
2
Fasoli S, Marzotto M, Rizzotti L. et al .
Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis.
Int J Food Microbiol.
2003;
82
59-70
3
Hamilton-Miller J M, Shah S, Winkler J T.
Public health issues arising from microbiological and labelling quality of foods and supplements containing probiotic microorganisms.
Public Health Nutr.
1999;
2
223-229
4
Temmerman R, Scheirlinck I, Huys G. et al .
Culture-independent analysis of probiotic products by denaturing gradient gel electrophoresis.
Appl Environ Microbiol.
2003;
69
220-226
8
Famularo G, De Simone C, Matteuzzi D. et al .
Traditional and high potency probiotic preparations for oral bacteriotherapy.
Biodrugs.
1999;
12
455-470
9
Sanders M E, Huis in’t Veld J H.
Bringing a probiotic-containing functional food to the market: microbiological, product, regulatory and labeling issues.
Antonie Van Leeuwenhoek.
1999;
76
293-315
10
Paubert-Braquet M, Gan X H, Gaudichon C. et al .
Enhancement of host resistance against Salmonella Typhimurium in mice fed a diet supplemented with yogurt or milks fermented with various Lactobacillus casei strains.
Int J Immunother.
1995;
11
153-161
11
Perdigon G, Nader de Macias M E, Alvarez S. et al .
Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus.
J Dairy Res.
1990;
57
255-264
12
Jin L Z, Ho Y W, Abdullah N. et al .
Effects of adherent Lactobacillus cultures on growth, weight of organs and intestinal microflora and volatile fatty acids in broilers.
Anim Feed Sci Technol.
1998;
70
197-209
13
Jin L Z, Ho Y W, Abdullah N. et al .
Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures.
Poult Sci.
2000;
79
886-891
14
Lema M, Williams L, Rao D R.
Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in lambs by feeding microbial feed supplement.
Small Rumin Res.
2001;
39
31-39
15
Zoppi G, Cinquetti M, Benini A. et al .
Modulation of the intestinal ecosystem by probiotics and lactulose in children during treatment with ceftriaxone.
Curr Ther Res.
2001;
62
418-435
17
Frohlich-Wyder M T, Bachmann H P, Casey M G.
Interaction between propionibacteria and starter/non-starter lactic acid bacteria in Swiss-type cheeses.
Lait.
2002;
82
1-15
19
Ouwehand A C, Isolauri E, Kirjavainen P V. et al .
The mucus binding of Bifidobacterium lactis Bb12 is enhanced in the presence of Lactobacillus GG and Lact. delbrueckii subsp. bulgaricus.
Lett Appl Microbiol.
2000;
30
10-13
21
Campieri M, Gionchetti P.
Probiotics in inflammatory bowel disease: new insight to pathogenesis or a possible therapeutic alternative?.
Gastroenterology.
1999;
116
1246-1249
23
Driessen F M, Kingma F, Stadhouder J.
Evidence that Lactobacillus bulgaricus in yoghurt is stimulated by carbon dioxide produced by Streptococcus thermophilus.
Neth Milk Dairy J.
1982;
36
135-144
24
Gomes A M, Malcata F X, Klaver F A.
Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk hydrolyzates.
J Dairy Sci.
1998;
81
2817-2825
25
Kailasapathy K, Chin J.
Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp.
Immunol Cell Biol.
2000;
78
80-88
26
Looijesteijn P J, Trapet L, de Vries E. et al .
Physiological function of exopolysaccharides produced by Lactococcus lactis.
Int J Food Microbiol.
2001;
64
71-80
27
Bello F D, Walter J, Hertel C. et al .
In vitro study of prebiotic properties of levan-type exopolysaccharides from lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis.
Syst Appl Microbiol.
2001;
24
232-237
28
Timmerman H M, Niers L E, Ridwan B U. et al .
Design of a multispecies probiotic mixture to prevent infectious complications in critically ill patients.
Clin Nutr.
2007;
26
450-459
29
Kalliomaki M, Salminen S, Arvilommi H. et al .
Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial.
Lancet.
2001;
357(9262)
1076-1079
30
Kopp M V, Hennemuth I, Heinzmann A. et al .
Randomized, double-blind, placebo-controlled trial of probiotics for primary prevention: no clinical effects of Lactobacillus GG supplementation.
Pediatrics.
2008;
121
e850-e856
32
Hahn E L, Bacharier L B.
The atopic march: the pattern of allergic disease development in childhood.
Immunol Allergy Clin North Am.
2005;
25
231-246
34
Wold A E.
The hygiene hypothesis revised: is the rising frequency of allergy due to changes in the intestinal flora.
Allergy.
1998;
53 (46 Suppl)
20-25
36
Holt P G, Upham J W, Sly P D.
Contemporaneous maturation of immunologic and respiratory functions during early childhood: implications for development of asthma prevention strategies.
J Allergy Clin Immunol.
2005;
116
16-24
37
Timmerman H M, Koning C J, Mulder L. et al .
Monostrain, multistrain and multispecies probiotics – A comparison of functionality and efficacy.
Int J Food Microbiol.
2004;
96
219-233
38
Niers L E, Timmerman H M, Rijkers G T. et al .
Identification of strong interleukin-10 inducing lactic acid bacteria which down-regulate T helper type 2 cytokines.
Clin Exp Allergy.
2005;
35
1481-1489
39
Niers L E, Hoekstra M O, Timmerman H M. et al .
Selection of probiotic bacteria for prevention of allergic diseases: immunomodulation of neonatal dendritic cells.
Clin Exp Immunol.
2007;
149
344-352
