DOI: 10.1055/s-00000017

Experimental and Clinical Endocrinology & Diabetes

Issue S 02 · Volume 104 · 1996 DOI: 10.1055/s-002-19242

Part I — Symposium and Plenary Lectures

1
Posner, Barry I.; Yale, Jean-Francois; Shaver, Alan: S-1: The Insulin Receptor Kinase (IRK)-associated Phosphotyrosine phosphatase (PTP): A key regulator of IRK function and a therapeutic target
2
Joshi, Rajiv L.; Deltour, Louise; Duvillié, Bertr; Cordonnier, Nathalie; Lamothe, Betty; Bucchini, Danielle; Jami, Jacques: S-2: Mice carrying null mutations in the insulin and insulin receptor genes
3
Accili, Domenico; Cola, Giovanni Di; Young Lee, C.; Cool, Martha H.: S-3: Metabolic abnormalities in mice lacking insulin receptors
4
Goldfine, Ira D.; Maddux, Betty A.; Youngren, Jack F.; Sbraccia, Paolo; Trischitta, Vincenzo; Dohm, G. Lynis: S-4: Insulin receptor inhibitors as a cause of insulin resistance
4
Efstratiadis, Argiris: S-5: Genetics of growth: Developmental roles of IGF and insulin receptors

Part II — Oral Presentations

Obesity and Adipocyte Metabolism

7
Hansen, B. C.; Hotta, K.; Bodkin, N.; Ortmeyer, H.; Nicolson, M.: O-1: The leptin-insulin axis: Interactions in the development of obesity and diabetes
8
Houseknecht, Karen L.; Flier, Sarah N.; Frevert, Ernst U.; Frederich, Robert C.; Flier, Jeffrey S.; Kahn, Barbara B.: O-2: Leptin secretion by the adipocyte: Regulation with genetic and dietary obesity and search for an intracellular storage pool
8
Michael Gibbs, E.; Zavadoski, William J.; Genereux, Paul E.; McGill, Paul D.; Lillquist, Jay S.; Etgen, Garret J.; Stevenson, Ralph W.; McCoid, Scott C.: O-3: Skeletal muscle expression of a human GLUTI transgene results in improved glucose homeostasis and weight loss in obese, diabetic (dbldb) mice
9
Echwald, Søren M.; Rasmussen, Susanne B.; Hansen, Lars; Tybjaerg-Hansen, Anne; Andersen, Teis; Sørensen, Thorkild I. A.; Pedersen, Oluf B.: O-4: Cloning and mutational analysis of the ob gene in juvenile onset obesity
10
Rahn, Tova; Resjö, Svante; Rönnstrand, Lars; Tornqvist, Hans; Manganiello, Vincent C; Belfrage, Per; Degerman, Eva: O-5: Identification of the site in the cGMP-inhibited phosphodiesterase phosphorylated in adipocytes in response to insulin and isoproterenol
11
Sun, Yang; Farrell, Craig J.; McCormack, Shana E.; Herron, Lisa M.; Yun, Jeung S.; Lechner, Pamela; Hakimi, Parvin; Hanson, Richard W.; Friedman, Jacob E.: O-6: Induction of PEPCK gene transcription in the genetically obese dbldb mouse occurs through a glucocorticoid response element

Receptor Structure & Function I

13
Garofalo, Robert S.; Chen, Chi; Marin, Mireya; Ruan, Yimin; Jack, Joseph: O-7: Drosophila insulin receptor function is required for normal growth
14
Lu, Kuanghui; Guidotti, Guido: O-8: Localization of the class I disulfide bonds of the human insulin receptor and the role of class I disulfide bonds in insulin receptor signal transduction
15
Dib, Karim; Navé, Barbara; Siddle, Kenneth: O-9: Characterisation of hybrid receptors in human fibroblasts

Receptor Structure & Function II

17
Krook, A.; Ouwens, D. M.; Moller, D. E.; Maassen, J. A.; O'Rahilly, S.: O-10: Mutant insulin receptors Arg1174Gln and Pro1178Leu — naturally occurring insulin receptor mutations as tools in the dissection of insulin signal transduction pathways
18
Bossenmaier, Birgit; Strack, Volker; Stoyanov, Borislav; Mushack, Joanne; Häring, Hans-Ulrich: O-11: Human insulin receptor (HIR)/IRS-1 interaction: Serines 1177/78/82 contribute to a second domain required for IRS-1 phosphorylation
19
Blakesley, VA; Esposito, D. L.; Scrimgeour, A. G.; Koval, A. P.; Stannard, B. S.; LeRoith, D.: O-12: Roles of the tyrosine residues in the carboxyterminal domain of the IGF-I receptor

Glucose Transport

21
Cheatham, Bentley; Volchuk, Allen; Kahn, C. Ronald; Wang, Lois; Rhodes, Christopher J.; Klip, Amira: O-13: Functional role of synaptobrevins in insulinstimulated translocation of GLUT4 glucose transporters
22
Kandror, K. V.; Pilch, P. F.: O-14: Protein composition of GLUT4-containing vesicles
22
Heller-Harrison, R. A.; Morin, M. M.; Guilherme, A.; Czech, M. P.: O-15: Specific targeting of PI 3-kinase to GLUT4 vesicles by insulin
24
Tosh, David; Pryor, Paul R.; Clark, Avril E.; Holman, Geoffrey D.: O-16: Accelerated GLUT4 endocytosis following chronic-insulin treatment of rat adipocytes
25
Tanti, Jean François; Grémeaux, Thierry; Ricort, Jean-Marc; Obberghen, Emmanuel Van; Marchand-Brustel, Yannick Le: O-17: Overexpression of a constitutively active form of Phosphatidylinositol 3 kinase is sufficient to Promote GLUT 4 translocation in transfected rat adipose cells
26
Cormont, Mireille; Bortoluzzi, Marie-Noëlle; Gautier, Nadine; Obberghen, Emmanuel Van; Marchand-Brustel, Yannick Le: O-18: Role of the small GTPases Rab4 and Rab5 in insulin-induced glucose transport
27
Shisheva, Assia; Czech, Michael P.: O-19: Increased cytosolic Rab4 mediated by insulin selectively associates with the GDI-1 isoform in 3T3-L1 adipocytes
29
Morris, Aaron J.; Martin, Stuart S.; Haruta, Tetsuro; Nelson, James G.; Gustafson, Thomas A.; Mueckler, Mike; Rose, David W.; Olefsky, Jerrold M.: O-20: Disruption of the interaction between the insulin receptor and IRS-1 does not impair insulin stimulated GLUT4 translocation
29
Giorgino, Francesco; Laviola, Luigi; Montrone, Carmela; Robertis, Ottilia de; McCowen, Karen; Smith, Robert J.: O-21: The carboxy-terminal domain of the GLUT4 glucose transporter interacts with a novel E2 ubiquitin-conjugating enzyme identified by two-hybrid cloning
31
Brozinick, J. T.; Reynolds, T. H.; Gibbs, E. M.; Cushman, S. W.: O-22: Regulation of glucose transport in skeletal muscle of rats and mice is mediated by the cell surface concentration of GLUT4
33
Hayashi, Hideki; Kishi, Kazuhiro; Kamohara, Seika; Tamaoka, Keisuke; Imanaka, Takanobu; Wang, Lihong; Mitani, Yasumasa; Ohnishi, Tetsuo; Ebina, Yousuke: O-23: Mechanisms of insulin-dependent and insulin-independent GLUT4 translocation

Postreceptor Signalling I

33
Almind, Katrine; Inoue, Gen; Pedersen, Oluf; Kahn, C. Ronal: O-24: A common amino acid polymorphism in IRS-1 causes impaired insulin signaling: Evidence from transfection studies
34
Sun, Xiao Jian; Wang, Ling-Mei; Zhang, Yitao; Yenush, Lynne; Glasheen, Erin; Myers, Martin G.; Lane, William S.; Pierce, Jacalyn H.; White, Morris F.: O-25: Purification and cloning of IRS-2 reveals the molecular bases of IRS-proteins in insulin and cytokine signaling
35
Conway, Bruce R.; Heller-Harrison, Robin A.; Sleeman, Mark W.; Czech, Michael P.: O-26: The pleckstrin homology (PH) domain of IRS-1 is required for tyrosine phosphorylation of IRS-1 but is not essential for membrane-localization or insulin-dependent release into cytosol
36
Sawka-Verhelle, Dominique; Tartare-Deckert, Sophie; White, Morris F.; Obberghen, Emmanuel Van: O-27: IRS-2 binds to the insulin receptor through its PTB domain and through a newly identified domain comprising amino acids 591 to 786
37
Yenush, Lynne; Myers, Martin G.; Grammer, Timothy C.; Blenis, John; White, Morris F.: O-28: Characterization of IRS-1 molecules containing single SH2-protein binding determinants
38
Krüger, Marianne Scheel; Klein, Harald; Siddle, Kenneth; Gammeltoft, Steen: O-29: Identical substrate specificity of insulin and IGF-1 receptor tyrosine kinases in phosphorylation of insulin receptor substrate-1
38
Mothe, I.; Van Obberghen, E.: O-30: Phosphorylation of IRS-1 on multiple serine residues, 612, 632, 662 and 731, modulates insulin action
39
Eldar-Finkeman, Hagit; Argast, Gretchen M.; Krebs, Edwin G: O-31: Expression of glycogen synthase kinase-3 mutants in cells and its effect on glycogen synthase activity
40
Pons, S.; White, M. F.: O-32: p55PIK is expressed and required during neuronal differentiation

Postreceptor Signalling II

42
Smith, Robert J; Chow, Jesse C; Condorelli, Gerolama: O-33: Tyrosine phosphorylation of Shc, but not IRS-1, requires IGF-I receptor internalization
44
Koval, A. P.; Blakesley, V. A.; Zick, Y.; LeRoith, D.: O-34: Characterization of the specific interaction of Crk with the IGF-I receptor
45
Ceresa, Brian P.; Pessin, Jeffrey E.: O-35: Insulin-mediated activation of STAT3
46
Kayali, Ayse; Haruta, Tetsuro; Saltiel, Alan; Olefsky, Jerrold; Webster, Nicholas J. G.: O-36: A possible role for phospholipase Cγ in insulin action?
47
Bernier, Michel; Kole, Hemanta K.: O-37: Specific inhibition of insulin receptor dephosphorylation enhances insulin signalling in intact cells
48
Goldstein, Barry J; Zhang, Wei-Ren; Ahmad, Faiyaz; Li, Pei-Ming: O-38: Regulation of insulin receptor function by the LAR protein-tyrosine phosphatase
49
Yamauchi, Keishi; Pessin, Jeffrey E.; Aizawa, Toru; Hashizume, Kiyoshi: O-39: Identification and characterization of SHPTP2-docking tyrosine-phosphorylated proteins
50
Ouwens, D. Margriet; Mikkers, Harald M. M.; van der Zon, Gerard C. M.; Stein-Gerlach, Matthias; Ullrich, Axel; Maassen, J. Antoine: O-40: Phosphotyrosine phosphatase 1D mediates insulin-induced tyrosine dephosphorylation of focal adhesion kinase and rearrangement of the actin cytoskeleton
51
Denton, Richard M.; Diggle, Tricia A.; Moule, S. Kelly; Avision, Matthew B.; Heesom, Kate J.; Flynn, Andrea; Proud, Christopher G.: O-41: Effects of insulin on the phosphorylation and dissociation of binding protein-1 (4E-BP1) from initiation factor 4E (eIF-4E) involves both rapamycin-sensitive and rapamycin-insensitive pathways
52
Ursø, Birgitte; Christoffersen, Claus T; Ouwens, Margriet; Vlahos, Chris; Tornqvist, Hans; White, Morris; Naor, David; Meyts, Pierre De: O-42: Mitogenic signalling by insulin in a T-cell lymphoma, the LB cell line, devoid of IGF-I receptors: evidence for the lack of involvement of the RAS-MAP kinase pathway and for a possibly novel IRS-like molecule
53
Harada, Shuko; Smith, Robert M.; Smith, Judith A.; White, Morris F.; Jarett, Leonard: O-43: Multiple pathways for insulin-induced egr-1 expression
55
Navé, B. T.; Hayward, A.; Siddle, K.; Shepherd, P. R.: O-44: Regulation of phosphoinositide 3-kinase by PDGF and insulin in 3T3-L1 adipocytes
57
Biener, Yael; Feinstein, Revital; Mayak, Michal; Kaburagi, Yasushi; Kadowaki, Takashi; Zick, Yehiel: O-45: Annexin II — a novel element in insulin signal transduction: role of annexin-II phosphorylation in insulin receptor internalization

Transgenic and Gene Knock-out Models

58
Yamauchi, Toshimasa; Tobe, Kazuyuki; Tamemoto, Hiroyuki; Kaburagi, Yasushi; Ueki, Kohjiro; Kadowaki, Takashi: O-46: Insulin signalling and insulin actions in the muscle, adipose tissue and liver of insulin resistant IRS-1 deficient mice

NIDDM and Insulin Resistance

59
Peraldi, Pascal; Hotamisligil, Gökhan S.; Buurman, Wim A.; White, M.; Spiegelman, Bruce M.: O-47: Mechanisms of TNF-α induced insulin resistance
59
Hurel, Stevn; Ofei, Francis; Wells, Ann; Newkirk, José; Sopwith, Mark; Turnbull, Douglas; Taylor, Roy: O-48: TNFα and insulin sensitivity in humans: effects in vivo of antibody blockade in obese NIDDM patients and in vitro upon human cultured myotubes
60
Andersen, C. B.; Hansen, T.; Echwald, S. M.; Urhammer, S. A.; Clausen, J. O.; Pedersen, O.: O-49: A prevalent amino acid polymorphism at codon 325Met->IIe in the gene encoding the phosphatidylinositol 3-kinase p85α regulatory subunit is associated with decreased glucose tolerance and glucose effectiveness
61
Kausch, C.; Hamann, A.; Niendorf, A.; Joost, H.-G.; Dreyer, M.; Rüdiger, H. W.; Holman, G. D.; Greten, H.; Matthaei, S.: O-50: A novel mechanism of cellular insulin resistance: Defective insulin-stimulated glucose transport due to malinsertion of glucose transporters into the plasma membrane of fibroblasts from a patient with an insulin resistance syndrome
63
Condorelli, Gerolama; Crescenzi, Elvira; Caiola, Agnese; Andreozzi, Francesco; Tocchetti, Carlo G.; Vigliotta, Giovanni; Tecce, Mario F.; Palumbo, Giuseppe; Beguinot, Francesco: O-51: Overexpression of the MAT-1 oncogene in non-insulin-dependent diabetes
64
Whitehead, J; Humphries, P; Krook, A; O'Rahilly, S: O-52: A naturally occurring mutation in IRS-1 responsible for severe insulin resistance in a human patient?

Part III — Posters

Obesity and Adipocyte Metabolism

66
Kroder, Gerhild; Kellerer, Monika; Häring, Hans Ulrich: P-1: Effect of leptin on insulin signalling in rat-1 fibroblasts overexpressing HIR
66
Baskin, Denis G.; White, Morris F.; Schwartz, Michael W.: P-2: Insulin and food intake increase neuronal phosphotyrosine levels in the arcuate nucleus of the rat hypothalamus
67
Lorenzo, M.; Valverde, A. M.; Teruel, T.; Navarro, R; Benito, M.: P-3: IGF-I/RAS induces growth and differentiation in brown adipocytes: a novel thermogenic mechanism
68
Bickel, Perry E.; Scherer, Philipp E.; Lodish, Harvey F.: P-4: Identification and cloning of adipocyte-specific secretory proteins

Receptor Structure and Function

68
Meyts, Pierre De; Christoffersen, Claus T.; Ursø, Birgitte; Pierreux, C. E.; Rousseau, Guy G.; Lemaigre, Frederic P.; Shymko, Ronald M.; Tornqvist, Hans: P-5: Insulin dose-response curves and IGF-I cross-reactivity in cells expressing only one receptor type: evidence for different requirements for monovalent versus bivalent insulin binding of metabolic and mitogenic signaling
70
Christoffersen, Claus T.; Larsø, Linda; Meyts, Pierre De; Tornqvist, Hans: P-6: Presence of inactive insulin-like growth factor-I receptors on primary rat adipocytes
71
Shymko, Ronald M.; Meyts, Pierre De: P-7: Kinetic and equilibrium properties of a cross-linking model of insulin receptor binding
72
Shymko, Ronald M.; Meyts, Pierre De; Thomas, René: P-8: Logical analysis of the effect of timing of receptor binding on signalling specificity
73
Shymko, Ronald M.; Dumont, Erik; Meyts, Pierre De; Dumont, Jacques E.: P-9: Analysis of determination of signalling specificity in the insulin receptor through coincidence of hormone and effector binding
74
Hammond, Bruce J.; Smith, Geoffrey D.: P-10: The crosslinking model of insulin receptor interactions: A compartmental analysis
75
Fußgänger, RD; Jehle, PM; Vetter, E; Saile, B; Kunze, U; Lutz, M; Schleyer, M; Warchol, W; Breitig, D: P-11: Proinsulin: Binding, signal transduction and biological effects
77
Bass, Joseph; Kristensen, Claus; Andersen, Asser S.; Wiberg, Finn C.; Schäffer, Lauge; Kjeldsen, Thomas; Steiner, Donald F.: P-12: Expression, characterization and purification of soluble insulin receptor-immunoglobulin chimeras that retain high-affinity insulin binding
78
Wallach, Brenda J.; Kjeldsen, Thomas; Shymko, Ronald M.; Meyts, Pierre De: P-13: The importance of the exon six encoded domain of the IR and IGF-I-R in ligand binding
78
Longo, Nicola; Langley, Sharon D.; Still, Maria J.; Elsas, Louis J.: P-14: Mutational analysis of the insulin receptor: Arginine 86 plays an essential role in insulin binding and stimulation of glucose transport
80
Soos, Maria A.; Siddle, Kenneth: P-15: The relationship between atypical IGF-1 receptors and classical receptors
81
Yakushiji, Fumiatus; Kristensen, Claus; Kjeldsen, Thomas; Andersen, Asser S.; Shymko, Ronald M.; Meyts, Pierre De: P-16: Kinetics and cooperative properties of insulin, IGF-I and a single chain insulin/IGF-I hybrid ligand (ICP) binding to a chimeric insulin/IGF-I receptor
82
Jui, H.-Y.; Goldsmith, P.; Accili, D.; Taylor, SI: P-17: Identification of a factor that stimulates autophosphorylation of insulin receptor-related receptor
83
Andersen, Asser S.; Wiberg, Finn C.; Siddle, Ken; Kjeldsen, Thomas: P-18: Increased insulin affinity of the IGF-I receptor requires a nonpolar aromatic amino acid at position #32
83
Hamer, I.; Paccaud, J.-P; Renfrew-Haft, C.; Taylor, S.; Carpentier, J.-L.: P-19: Dual role of a dileucine-centered motif in the control of insulin receptor internalization
84
Gill, Raj; Meyts, Pierre De; Pitts, Jim; Ursø, Birgitte; Wallach, Brenda; Wollmer, Axel: P-20: Receptor binding of IGF-1 requires a conformational change
86
Fabry, Marlies; Kleinjung, Jens; Thevis, Willi; Engels, Ulrike; Brandenburg, Dietrich: P-21: Studies on insulin and IGF-I receptors with specific probes of the ligands
87
Hodgson, D. R.; Daws, M. R.; Graya, J.; May, F. E. B.; Westley, B. R.: P-22: Conflicting activities of peptide analogues of the C and D domains of IGF-1 (JB-1 and JB-2)
88
Ward, C. W.; Sparrow, L. G.; Gorman, J. J.; Bentley, J. D.; Cosgrove, L.; Frenkel, M. J.; McKern, N. M.: P-23: Characterisation of the disulphide bonds and some oligosaccharide subunits of the human insulin receptor expressed in CHO-K1 cells
89
Schäffer, Lauge; Hansen, Per Hertz: P-24: Partial characterization of the disulfide bridges of the soluble insulin receptor
89
Flörke, R.-R.; Reinauer, H.: P-25: Cleavage of disulfide bonds can markedly stabilize the hormone-bound insulin receptor state Characterization of a novel class of receptor disulfide bonds
90
Hamer, I.; Philippe, J.; Zahnd, G.; Carpentier, J.-L.: P-26: Substitution of Arg 252 for cysteine in the insulin receptor leads to a conformational change of the α-subunit resulting in a decreased insulin binding
90
Jansson, Magnus; Hallén, Dan; Koho, Hannu; Nilsson, Björn; Kördel, Johan: P-27: Characterization of ligand binding properties of a soluble insulin-like growth factor receptor variant: Evidence for ligand induced structural changes
91
Hodgson, D. R.; May, F. E. B.; Westley, B. R.: P-28: Residues in insulin-like growth factor I involved in binding to the type I insulin-like growth factor receptor
91
Stoyanov, Borislav; Bossenmaier, Birgit; Mushack, Joanne; Häring, Hans-Ulrich: P-29: Serine phosphorylation sites 1293/94 and 1308/09 in the C-terminal end of the insulin receptor β-subunit modulate insulin dependent phosphorylation of an unidentified 66 kDa protein
92
Li, Shu-Lian; Siddle, Ken; Fujita-Yamaguchi, Yoko: P-30: The carboxyl terminal 110 amino acids of insulin-like growth factor-I receptor interact with the insulin receptor and activates its tyrosine protein kinase
93
Caruso, M.; Miele, C.; Formisano, P.; Bifulco, G.; Auriccio, R.; Oliva, A.; Oriente, F.; Polese, D.; Beguinot, F.: P-31: In skeletal muscle, expression of IR1152 decreases insulin-stimulated glucose disposal at the pyruvate dehydrogenase level
94
Kalloo-Hosein, H. E.; Whitehead, J.; Tavaré, J.; Siddle, K; O'Rahilly, S.: P-32: TrkC/insulin receptor and TrkC/IGF-1 receptor chimaeras: Tools for the examination of differential signalling to mitogenic and metabolic events by the intracellular domains of the insulin and IGF-1 receptors
96
Gardin, Anne; Auzan, Colette; Clauser, Eric; Malherbe, Tatiana; Meuillet, Emmanuelle; Crémel, Gérard; Hubert, Pierre: P-33: Substitution of the glycophorin A transmembrane domain modifies the structure of the insulin receptor and inhibits the action of insulin
97
Giannini, S.; Cresci, B.; Manuelli, C.; Pala, L.; Rotella, C. M.: P-34: An insulin-like growth factor-I receptor independent mechanism regulates insulin-like growth factor binding proteins in bovine retinal endothelial cells
99
Maggi, Davide; Andraghetti, Gabriella; Cordera, Renzo: P-35: CYS 860 in the insulin receptor ß subunit is critical for signal transduction in transfected CHO cells
100
Strack, V.; Bossenmaier, B.; Mushack, J.; Häring, H.-U.: P-36: 973 valine to methionine mutation of human insulin receptor (HIR): Functional characterisation in 293 cells
101
Ruiz, P.; Martinez, C.; Satrústegui, J.; Carrascosa, J. M.; Andrés, A.: P-37: Role of the C-terminal domain of insulin receptor β-subunit in aging: effects of truncation
102
Tahiri, K.; Chauvet, G.; Desbuquois, B.: P-38: Characterization of the molecular forms of the α and β subunits of the insulin receptor in endocytic compartments of rat liver

Glucose Transport

104
Malide, D.; Dwyer, N. K.; Blanchette-Mackie, E. J.; Cushman, S. W.: P-39: Dissection of the insulin responsive GLUT4 subcellular trafficking pathway in rat adipose cells using the PI3-kinase inhibitor wortmannin: A confocal microscopy approach
105
Shepherd, P. R.; Nave, B. T.; Nolte, L.; Rincon, J.; Galuska, D.; Siddle, K.; Zierath, J. R.; Wallberg-Henriksson, H.: P-40: Insulin stimulation of PI 3-kinase and MAP kinase and roles of these in glucose metabolism in human skeletal muscle
107
Dobson, Stephen P.; Livingston, Callum; Gould, Gwyn W.; Tavaré, Jeremy M.: P-41: Real-time analysis of insulin-stimulated GLUT4 translocation in single living cells using green fluorescent protein
107
Muñoz, Purificación; Mora, Silvia; Sevilla, Lidia; Kaliman, Perla; Tomàs, Eva; Gumà, Anna; Testar, Xavier; Palacín, Manuel; Zorzano, Antonio: P-42: Characterization of intracellular GLUT4 membrane populations in the muscle fiber. Caveolin does not colocalize with GLUT4 in intracellular membranes
108
Goodyear, Laurie J.; Hirshman, Michael F.; Thorell, Anders; Nygren, Jonas; Horton, Edward S.; Ljungqvist, Olle: P-43: GLUT4 translocation in human skeletal muscle: Responses to insulin infusion and surgical stress
111
Uphues, I.; Eckel, J.: P-44: Insulin-dependent glucose transport in cardiac muscle of obese Zucker rats: Studies on the role of small GTP-binding proteins
112
Wojtaszewski, Jørgen F. P.; Laustsen, Jan L.; Richter, Erik A.: P-45: Hypoxia- and contraction-stimulated glucose transport is affected differently by Wortmannin in perfused rat muscles
113
Zorzano, Antonio; Thomas, Julia; Muñoz, Purificación; Sevilla, Lidia; Holman, Geoffrey; Palacín, Manuel; Testar, Xavier; Kammermeier, Helmut; Fischer, Yvan: P-46: Insulin-induced recruitment of GLUT4 and GLUT1 glucose carriers in isolated rat cardiac myocytes. Evidence for the existence of different intracellular GLUT4 vesicle populations
114
Dabur, Z.; Armoni, M.; Zlozover, M.; Burvin, R.; Harel, C.; Karnieli, E.: P-47: The effects of thiamine (vitamin B1) on glucose uptake
115
Pither, Richard; Game, Stephen; Davis, Julie; Katz, Marion; McLane, John: P-48: The use of Cytostar-T™ scintillating microplates to monitor insulin-dependent glucose uptake by 3T3-L1 adipocytes

Postreceptor Signalling

116
Niederfellner, Gerhard; Miyaji, Hiromasa; White, Morris F.: P-49: Interaction of IRS molecules with a novel 19 kD protein that carries an armadillo motif
117
Kanety, Hannah; Hemi, Rina; Papa, Moshe; Karasik, Avraham: P-50: Sphingomyelinase and ceramide mimic TNF-α inhibitory effect on insulin-induced tyrosine phosphorylation of IRS-1
118
Tornqvist, Hans; Amstrup, Jan; Seedorf, Klaus: P-51: Patterns of IRS-1 phosphorylation and interaction with SH2 domain containing proteins in response to insulin, IGF-1, growth hormone and cytosolic tyrosine kinases
118
Taouis, Mohammed; Derouet, Michel; Simon, Jean; Taylor, Simeon I.; Reitman, Marc: P-52: Characterisation of insulin signalling in chicken
119
Murer, Erik H.; Martinez, Nestor J.; Obley, Daniel: P-53: Platelet activation modulated by Vanadate, Pervanadate and Wortmannin; role of „crosstalk”
121
Najjar, S. M.; Li Calzi, S.; Lakkis, M.; Nabih, Z. T.; Choice, C. V.: P-54: pp 120, a specific substrate of the insulin receptor tyrosine kinase, differentially regulates internalization of two variant isoforms of the insulin receptor
122
Perfetti, Riccardo; Lee-Kwon, Whaseon; Montrose-Rafizadeh, Chahrzad; Bernier, Michel: P-55: Activation of insulin receptor tyrosine kinase enhances DNA repair
123
Pierreux, C. E.; Ursø, B.; Aidant, N.; De Meyts, P.; Rousseau, G. G.; Lemaigre, F. P.: P-56: Signaling pathway leading to the insulin-induced inhibition of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene transcription
123
Roder, Karim; Wolf, Siegmund S.; Schweizer, Michael: P-57: The role of Sp1 in the diet-induced regulation of the rat fatty acid synthase (FAS) gene
124
Sell, Susan M.; Reese, David; Leduc, Richard: P-58: Evidence for hormone-induced differential splicing of PTP1B in vivo
125
Standaert, Mary L.; Karnam, Purushotham; Bandyopadhyay, Gautam; Galloway, Lamar; Farese, Robert V.: P-59: Insulin-sensitive phospholipid signalling systems and their relation to glucose transport
126
Wertheimer, E.; Tennenbaum, T.; Graham, D. E.; Barth, K.; Yuspa, S. H.; Taylor, S. I.: P-60: The role of the insulin receptor in cellular growth and tumorigenesis
126
Yu, Zhi-Wen; Eriksson, Jan W.: P-61: Opposing effects of insulin and cAMP on GLUT4 gene expression in 3T3-F442A cells
127
Sánchez-Margalet, Victor; Goldfine, Ira D.; Sung, Chin K.: P-62: Role of phosphatidylinositol-3-kinase and S6 kinase in insulin-stimulated glycogen synthesis
128
Klarlund, Jes K.; Cherniack, Andrew D.; McMahon, Martin; Czech, Michael P.: P-63: Activation of the Raf/Mitogen-activated protein kinase pathway is not sufficient for disassembly of Grb2/Sos or p21ras desensitization
129
Moule, Kelly; Edgell, Nigel; Welsh, Gavin; Foulstone, Emily; Denton, Richard: P-64: Multiple signalling pathways involved in the metabolic actions of insulin in rat epididymal fat cells
131
Virbasius, Joseph V.; Guilherme, Adilson; Czech, Michael P.: P-65: Phosphatidylinositol 3-kinases of adipocytes include enzymes structurally and biochemically distinct from p110
132
Ogawa, Wataru; Sakaue, Hiroshi; Takada, Masafumi; Kuroda, Shyoji; Kotani, Ko; Ueno, Hikaru; Kasuga, Masato: P-66: Introduction of dominant negative molecules into 3T3L1 adipocytes using adenovirus vector
132
Gnudi, Luigi; Frevert, Ernst U.; Houseknecht, Karen L.; Kahn, Barbara B.: P-67: Use of recombinant adenovirus-mediated gene transfer to investigate the effects of dominant negative ras on the activation of PI3 kinase and glucose transport in 3T3L1 adipocytes
133
Berhanu, Paulos; Anderson, Celia; Wood, William M.; Ciaraldi, Theodore P.: P-68: Evidence for mitogenic signaling by the insulin receptor kinase independent of Shc phosphorylation
135
Hayward, A. C.; Beeton, C. A.; Shepherd, P. R.; Siddle, K.: P-69: Stable expression of signalling proteins in 3T3-L1 adipocytes
136
Burnol, A.-F; Perdereau, D.; Kasus-Jacobi, A.; Girard, J.: P-70: Identification of new substrates of the insulin receptor tyrosine kinase
136
Grunberger, G.; Srinivas, P. R.; Mathews, S. T.; Leon, M. A.; Goustin, A. S.: P-71: α2-HS glycoprotein inhibits insulin-stimulated insulin receptor signaling in vitro and in vivo
138
Seedorf, Klaus; Juhl, Lone; Hansen, Lone; Nielsen, Lene; Mosthaf, Luitgard: P-72: Insulin-induced activation of MAP kinase involves GRB-2, SHPTP2, PI-3 kinase and PLCγ
139
Kitamura, Yukari; Ogawa, Wataru; Kitamura, Tadahiro; Kasuga, Masato: P-73: Identification of Napl (Nck-associated protein 1) as a target of activated GTP-binding protein Rac
139
Wijkander, Jonny; Rahn, Tova; Castan, Isabelle; Stenson-Holst, Lena; Manganiello, Vincent; Belfrage, Per; Degerman, Eva: P-74: Mitogen-activated protein kinase and p70 ribosomal S6 kinase are not involved in the insulin-mediated phosphorylation of cGMP-inhibited phosphodiesterase
140
Krause, Ulrike; Rider, Mark H.; Hue, Louis: P-75: Involvement of protein kinases of the insulin signalling pathway in the activation of glycogen synthase and acetyl-CoA carboxylase by liver cell swelling
141
Cosgrove, L. J.; Gough, K. H.; Macaulay, S. L.; Rashke, M.; Wan, S. S.; Tribbick, G.; Wang, J.; Ward, C. W.: P-76: Systematic mapping of potential binding sites for SHC and GRB2 SH2-domains on IRS-1 and the receptors for insulin, EGF, EGF, PDGF and FGF
141
Srivastava, A. K.; Chiasson, J.-L.; Anand-Srivastava, M. B.; Pandey, S. K.: P-77: Insulinomimetic agent, vanadyl sulfate (VS)-stimulated phosphatidylinositol 3-kinase (PI 3-K) and RAS/MAP kinase activation is independent of insulin receptor (IR) tyrosyl phosphorylation and is blocked by Wortmannin
142
Sung, Chin K.; Sanchez-Margalet, Victor; Choi, Woo S.: P-78: Role of p85 isoforms of phosphatidylinositol-3-kinase in insulin receptor signaling
142
Wasner, H. K.; Amini-Hadi-Kiashar, H.; Gypakis, A.; Niktopoulos, A.: P-79: Cyclic PIP synthetase, activated by tyrosine phosphorylation, combines prostaglandin E with activated inositol phosphate to cyclic PIP, a mediator of insulin action
143
Worm, Dorte; Vinten, Jørgen; Stæhr, Peter; Henriksen, Jan Erik; Handberg, Aase; Beck-Nielsen, Henning: P-80: The effect of insulin on phosphotyrosine phosphatase activity in skeletal muscle fractions from NIDDM patients and control subjects
143
Olivier, A.; Katz, S.; Burn, P.; Wertheimer, S.: P-81: Interaction between SH-PTP2 (SYP) and the insulin receptor is phosphorylation dependent
144
Zoppini, G.; Galante, P.; Muggeo, M.: P-82: Insulin induces tyrosine dephosphorylation of a 92 kDa protein in suspended monocytes
144
Shechter, Yoram: P-83: Alternative pathways of manifesting the rapidmetabolic effects of insulin
145
Gammeltoft, Steen; Frödin, Morten; Grønborg, Mette; Holm, Ninna R.; Christophersen, Palle; Olesen, Søren P.: S-6: Neurotrophin signaling in developing and differentiating neurons
145
Algenstaedt, Petra; Antonetti, David A.; Ronald Kahn, C.: P-124: Novel interactions of IRS-1 in human skeletal muscle

Gene Expression

146
Barth, S.; Hartmann, M.; Hahn, T.; Cervar, M.; Sesti, G; Kaufmann, P.; Techner, R.; Desoye, G.: P-84: Distribution of insulin receptor isoforms in the trophoblast of first trimester and term human placenta
147
Barth, S.; Klein, W.; Friehs, I.; Rigler, B.; Zechner, R.; Gasser, R.: P-85: GLUT4 mRNA expression in human myocardium is altered in patients with non-insulin-dependent diabetes mellitus
148
Barth, S.; Klein, W.; Köppel, H.; Petnehazy, T.; Pieber, T.; Friehs, I.; Rigler, B.; Zechner, R.; Gasser, R.: P-86: GLUT4 mRNA expression in human myocardium does not correlate with coronary heart disease
148
Bortoli, S.; Collinet, M.; Desbuquois, B.; López, S.: P-87: Regulation of insulin receptor gene expression in rat hepatoma cells: Effects of insulin, vanadate and cAMP
149
Cooper, Denise R.; Chalfant, Charles E.; Watson, James E.; Patel, Niketa; Yamamoto, Mayumi; McLean, Mark; Mischak, Harald; Eichler, Duane: P-88: Insulin-induced changes in alternative splicing of MRNA: A mechanism for enhancing insulin responsiveness
150
Kotzka, J.; Streicher, R.; Siemeister, G.; Knebel, B.; Munck, M.; Krone, W.; Müller-Wieland, D.: P-89: Regulation of the LDL receptor gene by insulin
151
Laurell, Henrik; Grober, Jacques; Lacombe, Thierry; Dauzats, Michèle; Holm, Cecilia; Langin, Dominique: P-90: Exon skipping leads to a catalytically inactive form of hormone-sensitive lipase in human adipose tissue
152
Lighten, A. D.; Hardy, K.; Moore, G. E.; Winston, R. M. L.: P-91: Expression of insulin-like growth factors in the human preimplantation embryo
153
Macaulay, S. L.; Cosgrove, L.; Hewish, D. R.; Gough, K. H.; Jagadish, M.; Frenkel, M. J.; Grusovin, J.; Verkuylen, A.; Fernandez, C. S.; Alafaci, A. M.; MacPherson, S. F.; Stoichevska, V.; Ward, C. W.: P-92: Expression of the synaptic vesicle core protein SNAP-25 in insulin responsive cells

Animal Models

154
Amessou, M.; Fouque, F.; Soussi, N.; Desbuquois, B.; Benelli, C.: P-93: Pretranslational regulation of pyruvate dehydrogenase complex E1α subunit in liver from genetically obese Zucker rats
155
Dahri, S.; Gego, C.; Grégoire, F.; Reusens, B.; Remacle, C.; Hoet, J. J.: P-94: Effect of isocaloric low protein diet during gestation on IGFBPs content in serum and organs of mother and fetus in rat
155
Daugaard, Jens R.; Laustsen, Jan L.; Hansen, Birgit Sehested; Richter, Erik A.: P-95: No effect of growth hormone treatment on insulin action in muscle of growth hormone deficient rats
156
Escrivá, F.; Agote, M.; Molero, J. C.; Pascual-Leone, A. M.; Andrés, A.; Satrústegui, J.; Carrascosa, J. M.: P-96: Changes in insulin-dependent glucose uptake by specific tissues of the aged Wistar rat
157
Incerpi, Sandra; Rizvi, S. Ibrahim; Vito, Paolo De; Luly, Paolo: P-98: Different pathways of Na/H antiport stimulation by insulin in the course of differentiation from myoblasts to myotubes
158
Kasuya, Junko; Fujita-Yamaguchi, Yoko: P-99: Regulation of cAMP phosphodiesterase and DNA synthesis by glucose and insulin in rat vascular smooth muscle cells
160
Valérie, Nivet; Jean-Pierre, Clot; Sophie, Ellouk-Achard; Geneviève, Descamps; Bernard, Desbuquois; Dominique, Durand: P-100: Expression of liver protein kinase C (PKC) α is increased in streptozotocin-induced diabetic rats but activity of calcium-dependent PKC is unchanged
161
Schmitz-Peiffer, Carsten; Browne, Carol L.; Oakes, Nicholas D.; Watkinson, Allan; Chisholm, Donald J.; Kraegen, Edward W.; Biden, Trevor J.: P-101: Alterations in the expression and cellular localization of protein kinase C isozymes are associated with insulin resistance in skeletal muscle of the high-fat-fed rat
162
Shah, Bukhtiar H.; Memon, R. A.; Ren, Y.; Sagar, G. D.; Hausman, R. E.: P-102: Role of insulin in the cholinergic and GABAergic neurotransmitter differentiation in the developing retina
164
Zakrzewska, Katherine; Cusin, Isabelle; Rohner-Jeanrenaud, Françoise; Shafrir, Eleazar; Jeanrenaud, Bernard: P-103: Metabolic adaptation to a low energy intake in Psammomys (sand rat)
164
Ziv, Ehud; Kalman, Rony; Bar-On, Hanoch; Shafrir, Eleazar: P-104: Potentation of insulin action by vanadyl treatment of diabetic Psammomys obesus

NIDDM and Insulin Resistance

166
Desbois-Mouthon, C.; Magré, J.; Sert-Langeron, C.; Blivet-Van Eggelpoël, M. J.; Besmond, C.; Capeau, J.; Caron, M.: P-105: Altered insulin receptor functions due to different mutations in the insulin receptor gene in four patients with leprechaunism
167
Hansen, Bo F.; Danielsen, Gillian M.; Vissing, Henrik; Goding, James W.; Lundemose, Anker G.: P-106: PC-1 overexpression does not affect insulin action in Chinese hamster ovary cells
168
Hansen, Lars; Hansen, Torben; Clausen, Jesper; Echwald, Søren M.; Pedersen, Oluf: P-107: A common polymorphism in the insulin receptor gene is a marker of non-insulin dependent diabetes mellitus and reduced first-phase insulin secretion in a Danish population of Caucasians
169
Hansen, T.; Eiberg, H.; Urhammer, S. A.; Pedersen, O.: P-108: Phenotype characteristics of two Danish MODY families with linkage to chromosome 12
170
Humphreys, P. J.; Dib, K.; Kumar, S.; Harvey, T.; O'Rahilly, S.: P-109: Impaired activation of phosphatidyl-inositol-3-kinase by insulin in cultured dermal fibroblasts from patients with severe insulin resistance and pseudo-acromegaly
171
Klein, Harald H.; Müller, Regina; Drenckhan, Maren; Vestergaard, Henrik; Pedersen, Oluf: P-110: Measurement of insulin receptor kinase activity in muscle and erythrocytes of a family with mutations of the insulin receptor gene
173
Knebel, B.; Kotzka, J.; Siemeister, G.; Schiller, M.; Kellner, S.; Dreyer, M.; Seemanova, E.; Krone, W.; Müller-Wieland, D.: P-111: Defects of insulin and IGF-I action at postreceptor level in patients with inherited syndromes of insulin resistance
174
Liu, L.; Till, M.; Niggemann, J.; Hauner, H.; Eckel, J.: P-112: TNF-alpha-induced insulin resistance in human adipocytes
175
Magré, J.; Vigouroux, C.; Desbois-Mouthon, C.; Hilbert, P.; Fauré, S.; Gedde Dahl, T.; Weissenbach, J.; Capeau, J.; Malderghem, L. Van; Berardinelli-Seip Study Group: P-113: Search for the gene(s) responsible for lipoatrophic diabetes
176
Memon, Riaz A.; Shah, B. H.; Grunfeld, C.; Feingold, K. R.: P-114: Role of intestinal lipogenesis in the hyperlipidemia of non-insulin dependent diabetes mellitus
178
Møller, A. M.; Hansen, T.; Almind, K.; Inoue, H.; Ferrer, J.; Permutt, A.; Urhammer, S. A.; Echwald, S. M.; Hansen, L.; Clausen, J. O.; Pedersen, O.: P-115: Sequence variants in the sulfonylurea receptor gene are associated with non-insulin dependent diabetes mellitus and with decreased insulin secretion after an intravenous tolbutamide challenge
178
Thye-Rønn, P.; Knudsen, C.; Levin, K.; Mencke, Steen; Gjessing, H. J.; Beck-Nielsen, H.: P-116: Physical fitness, body composition and intraabdominal fat: Relation to insulin resistance and insulin secretion in relatives of patients with NIDDM
179
Trischitta, V.; Youngren, J.; Frittitta, L.; Vigneri, R.; Goldfine, I. D.: P-117: Relationship between insulin sensitivity, insulin receptor tyrosine-kinase activity and PC-1 content
181
Urhammer, Søren A.; Clausen, Jesper O.; Hansen, Torben; Pedersen, Oluf: P-118: Insulin sensitivity and body weight changes in young Caurcasian carriers of the codon 64 amino acid polymorphism of the β3-adrenergic receptor gene
181
Vaag, Allan; Populsen, Pernille; Kyvik, Kirsten O.; Beck-Nielsen, Henning: P-119: Etiology of NIDDM: Genetics versus pre- or post natal environments? Results from twin studies
182
Youngren, J. F.; Goldfine, I. D.; Pratley, R. E.: P-120: Insulin resistance is related to defects in insulin receptor tyrosine kinase activation in Pima Indians
184
Yu, Zhi-Wen; Jansson, Per-Anders; Eriksson, Jan W.: P-121: Effects of peroxovanadate and insulin on lipolysis and glucose uptake in adipocytes from NIDDM patients
185
Mechin, Maire-Claire; Rider, Mark H.; Hue, Louis: P-122: Mechanism of stimulation of glycolysis by insulin in isolated cardiomyocytes