Übersicht über Einsatzmöglichkeiten von In-house-Tests zum Nachweis von systemischen Infektionskrankheiten beim Hund

Zusammenfassung

Zum Nachweis von Infektionskrankheiten existieren neben den in kommerziellen Laboratorien durchführbaren Verfahren auch sogenannte Point-of-care- (POC-) oder In-house-Tests. Diese basieren auf einem Enzyme-Linked Immunosorbent Assay (ELISA) oder anderen immunchromatografischen Testprinzipien und liefern binnen weniger Minuten direkt vor Ort ein Testergebnis. Dieser Artikel gibt einen Überblick über die Qualität der zahlreichen POC-Tests verschiedener Hersteller zum Nachweis von Parvovirus-Antigen im Kot, Antigen von Dirofilaria (D.) immitis im Blut sowie zum Nachweis von Antikörpern gegen Borrelia (B.) burgdorferi, Anaplasma (A.) spp., Ehrlichia (E.) spp., Leptospira (L.) spp. und Leishmania (L.) infantum im Blut (einzeln oder in Kombinationen). Entscheidend für den Nutzen der Tests sind ihre Sensitivität und Spezifität. Auch muss das Vorhandensein von Antikörpern oder der Nachweis von Antigen mit dem Auftreten einer klinischen Erkrankung einhergehen. POC-Tests zum Nachweis von Parvovirus-Antigen im Kot besitzen zwar eine sehr hohe Spezifität, die Sensitivität aller evaluierten Tests ist jedoch sehr niedrig. Die POC-Tests zum Nachweis von D. immitis besitzen eine sehr hohe Sensitivität und Spezifität. Da sie Antigen aus dem Uterus adulter, weiblicher Tiere nachweisen, ist der Test jedoch negativ, wenn keine oder zu wenig adulte, weibliche Dirofilarien vorhanden sind. Der POC-Test zum Nachweis von B.-burgdorferi-Antikörpern zeigt nur den Erregerkontakt an und nicht die klinische Erkrankung, da das Auftreten von Antikörpern nur in seltensten Fällen mit einer Erkrankung einhergeht. Ebenso ist der POC-Test zum Nachweis von Antikörpern gegen A. phagocytophilum zur Diagnose einer klinischen Anaplasmose ungeeignet, da auch diese Infektion nur selten zur Erkrankung führt und wenn, dann Symptome in der Regel vor Ausbildung der Antikörper auftreten. Zum Nachweis von Antikörpern gegen E. canis zeigen die POC-Tests eine sehr hohe Sensitivität und Spezifität. POC-Tests zum Nachweis von Antikörpern gegen L. infantum und Leptospira-Spezies (spp.) besitzen ebenfalls eine sehr hohe Spezifität und eine hohe Sensitivität. Jedoch kann es bei Letzteren zu positiven Ergebnissen nach einer Impfung kommen, da die Tests nicht zwischen Feld- und Impfantikörpern unterscheiden können.

Abstract

For detection of infectious diseases, several point-of-care (POC) tests are on the market in addition to methods performed in commercial laboratories. These POC tests are based on enzyme-linked immunosorbent assay (ELISA) or other immunochromatographic technologies and present results within few minutes in veterinary practice. This article gives an overview of the utility of numerous POC tests of different manufacturers for detection of parvovirus antigen in feces, Dirofilaria (D.) immitis antigen in blood as well as antibodies against Borrelia (B.) burgdorferi, Anaplasma (A.) spp., Ehrlichia (E.) spp., Leptospira (L.) spp. and Leishmania (L.) infantum in blood (single or in different combinations). Sensitivity and specificity of these tests are important for their usefulness in veterinary practice. Furthermore, presence of antibodies or detection of antigen has to correlate with the presence of clinical signs. POC tests for detection of canine parvovirus antigen have a very high specificity, the sensitivity of all evaluated POC tests, however, is very low. POC tests for detection of D. immitis antigen have a very high sensitivity and specificity. As they detect antigen from the uterus of female adult parasites, test results are negative when only very few female or only male adults are present. POC tests for detection of antibodies against B. burgdorferi only indicate contact with Borrelia spp. and do not prove clinical Lyme disease, as the infection only extremely rarely causes clinical signs. POC tests for detection of antibodies against A. phagocytophilum are also not suitable for diagnosis of clinical anaplasmosis. Infections with A. phagocytophilum only lead to clinical disease in very rare cases and in these, clinical signs occur before the development of antibodies. POC tests for detection of antibodies against E. canis have a very high sensitivity as well as specificity. POC tests for detection of antibodies against L. infantum and Leptospira species (spp.) show a very high specificity and a high sensitivity. However, Leptospira spp. antibody-positive results may occur following vaccination, as the POC tests cannot distinguish between field and vaccination strains.

Schlüsselwörter

Kanine - Schnelltest - Point-of-care-Test - Antigen - Antikörper

Keywords

Canine - point-of-care test - antigen - antibody

Publikationsverlauf

Eingereicht: 24. April 2023

Angenommen: 06. August 2023

Artikel online veröffentlicht:
03. Mai 2024

• Literatur

• 1 Beelitz P, Pfister K. Diagnostik und Therapie von Reisekrankheiten beim Hund. Tierarztl Prax Ausg K Kleintiere Heimtiere 2004; 32: 158-165 DOI: 10.1055/s-0037-1622411.
• 2 Knipper A. Neue gefährliche Parasiten werden in Deutschland heimisch. team spiegel 2006; 13: 7-8 DOI: 10.1055/s-0029-1237189.
• 3 Selbitz H-J, Truyen U, Valentin-Weigand P. Tiermedizinische Mikrobiologie, Infektions-und Seuchenlehre. Stuttgart: Georg Thieme Verlag; 2015. DOI: 10.1055/b-002-5219
• 4 Little SE. Ehrlichiosis and anaplasmosis in dogs and cats. Vet Clin North Am Small Anim Pract 2010; 40: 1121-1140 DOI: 10.1016/j.cvsm.2010.07.004.
• 5 O’Connor TP. SNAP assay technology. Top Companion Anim Med 2015; 30: 132-138 DOI: 10.1053/j.tcam.2015.12.002.
• 6 Chu K. An introduction to sensitivity, specificity, predictive values and likelihood ratios. Emerg Med 1999; 11: 175-181 DOI: 10.1046/j.1442-2026.1999.00041.x.
• 7 McCandlish IA, Thompson H, Fisher E. et al. Canine parvovirus infection. In Pract 1981; 3: 5-14 DOI: 10.1136/inpract.3.3.5.
• 8 Parrish CR, Aquadro CF, Strassheim M. et al. Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 1991; 65: 6544-6552 DOI: 10.1128/jvi.65.12.6544-6552.1991.
• 9 Goddard A, Leisewitz AL. Canine parvovirus. Vet Clin North Am Small Anim Pract 2010; 40: 1041-1053 DOI: 10.1016/j.cvsm.2010.07.007.
• 10 Buonavoglia C, Martella V, Pratelli A. et al. Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 2001; 82: 3021-3025 DOI: 10.1099/0022-1317-82-12-3021.
• 11 Hoskins JD. Update on canine parvoviral enteritis. Vet Med 1997; 92: 694-709
• 12 Decaro N, Buonavoglia C. Canine parvovirus—a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 2012; 155: 1-12 DOI: 10.1016/j.vetmic.2011.09.007.
• 13 Desario C, Decaro N, Campolo M. et al. Canine parvovirus infection: which diagnostic test for virus?. J Virol Methods 2005; 126: 179-185 DOI: 10.1016/j.jviromet.2005.02.006.
• 14 Decaro N, Elia G, Martella V. et al. A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs. Vet Microbiol 2005; 105: 19-28 DOI: 10.1016/j.vetmic.2004.09.018.
• 15 Decaro N, Desario C, Billi M. et al. Evaluation of an in-clinic assay for the diagnosis of canine parvovirus. Vet J 2013; 198: 504-507 DOI: 10.1016/j.tvjl.2013.08.032.
• 16 Decaro N, Desario C, Beall MJ. et al. Detection of canine parvovirus type 2c by a commercially available in-house rapid test. Vet J 2010; 184: 373-375 DOI: 10.1016/j.tvjl.2009.04.006.
• 17 Schmitz S, Coenen C, Matthias K. et al. Comparison of three rapid commercial canine parvovirus antigen detection tests with electron microscopy and polymerase chain reaction. J Vet Diagn Invest 2009; 21: 344-345 DOI: 10.1177/%2F104063870902100306.
• 18 Walter-Weingärtner J, Bergmann M, Weber K. et al. Comparison of Eight Commercially Available Faecal Point-of-Care Tests for Detection of Canine Parvovirus Antigen. Viruses 2021; 13: 2080 DOI: 10.3390/v13102080.
• 19 Proksch A, Unterer S, Speck S. et al. Influence of clinical and laboratory variables on faecal antigen ELISA results in dogs with canine parvovirus infection. Vet J 2015; 204: 304-308 DOI: 10.1016/j.tvjl.2015.03.009.
• 20 Macartney L, McCandlish I, Thompson H. et al. Canine parvovirus enteritis 2: Pathogenesis. Vet Rec 1984; 115: 453-460 DOI: 10.1136/vr.115.18.453.
• 21 Meunier P, Cooper B, Appel M. et al. Pathogenesis of canine parvovirus enteritis: the importance of viremia. Vet Pathol 1985; 22: 60-71 DOI: 10.1177/%2F030098588502200110.
• 22 Decaro N, Crescenzo G, Desario C. et al. Long-term viremia and fecal shedding in pups after modified-live canine parvovirus vaccination. Vaccine 2014; 32: 3850-3853 DOI: 10.1016/j.vaccine.2014.04.050.
• 23 Chandrashekar R, Mainville CA, Beall MJ. et al. Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs. Am J Vet Res 2010; 71: 1443-1450 DOI: 10.2460/ajvr.71.12.1443.
• 24 Otranto D, Capelli G, Genchi C. Changing distribution patterns of canine vector borne diseases in Italy: leishmaniosis vs. dirofilariosis. Parasit Vectors 2009; 2: 1-8 DOI: 10.1186/1756-3305-2-S1-S2.
• 25 Morchón R, Moya I, González-Miguel J. et al. Zoonotic Dirofilaria immitis infections in a province of Northern Spain. Epidemiol Infect 2010; 138: 380-383 DOI: 10.1017/S0950268809990434.
• 26 Vieira AL, Vieira MJ, Oliveira JM. et al. Prevalence of canine heartworm (Dirofilaria immitis) disease in dogs of central Portugal. Parasite 2014; 21 DOI: 10.1051/parasite/2014003.
• 27 Pantchev N, Norden N, Lorentzen L. et al. Current surveys on the prevalence and distribution of Dirofilaria spp. in dogs in Germany. Parasitol Res 2009; 105: 63-74 DOI: 10.1007/s00436-009-1497-7.
• 28 Lefkaditis M, Koukeri S, Cozma V. An endemic area of Dirofilaria immitis seropositive dogs at the eastern foothills of Mt Olympus, Northern Greece. Helminthologia 2010; 47: 3-7 DOI: 10.2478/s11687-010-0001-3.
• 29 Simsek S, Utuk A, Koroglu E. et al. Serological and molecular studies on Dirofilaria immitis in dogs from Turkey. J Helminthol 2008; 82: 181-186 DOI: 10.1017/S0022149X0896079X.
• 30 Ciucâ L, Musella V, Miron LD. et al. Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of eastern Romania. Geospat Health 2016; 11 DOI: 10.4081/gh.2016.499.
• 31 Panayotova-Pencheva M, Mirchev R, Trifonova A. Dirofilaria immitis infection in carnivores from Bulgaria: 2012–2013 update. Bulg J Vet Med 2016; 19: 153-162 DOI: 10.15547/bjvm.918.
• 32 Jacsó O, Mándoki M, Majoros G. et al. First autochthonous Dirofilaria immitis (Leidy, 1856) infection in a dog in Hungary. Helminthologia 2009; 46: 159-161 DOI: 10.2478/s11687-009-0030-y.
• 33 Tasić A, Rossi L, Tasić S. et al. Survey of canine dirofilariasis in Vojvodina, Serbia. Parasitol Res 2008; 103: 1297-1302 DOI: 10.1007/s00436-008-1132-z.
• 34 Mrljak V, Kuleš J, Mihaljević Ž. et al. Prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in Croatia. Vector Borne Zoonotic Dis 2017; 17: 398-408 DOI: 10.1089/vbz.2016.1990.
• 35 Svobodová Z, Svobodová V, Genchi C. et al. The first report of authochthonous dirofilariosis in dogs in the Czech Republic. Helminthologia 2006; 43: 242-245 DOI: 10.2478/s11687-006-0046-5.
• 36 Hamel D, Silaghi C, Knaus M. et al. Detection of Babesia canis subspecies and other arthropod-borne diseases in dogs from Tirana, Albania. Wien Klin Wochenschr 2009; 121: 42-45 DOI: 10.1007/s00508-009-1234-3.
• 37 Panarese R, Iatta R, Mendoza-Roldan JA. et al. Comparison of diagnostic tools for the detection of Dirofilaria immitis infection in dogs. Pathogens 2020; 9: 499 DOI: 10.3390/pathogens9060499.
• 38 Courtney CH, Zeng Q-Y. Relationship between microfilaria count and sensitivity of the direct smear for diagnosis of canine dirofilariosis. Vet Parasitol 2001; 94: 199-204 DOI: 10.1016/S0304-4017(00)00377-0.
• 39 Henry LG, Brunson KJ, Walden HS. et al. Comparison of six commercial antigen kits for detection of Dirofilaria immitis infections in canines with necropsy-confirmed heartworm status. Vet Parasitol 2018; 254: 178-182 DOI: 10.1016/j.vetpar.2018.02.037.
• 40 Trancoso TAL, Lima NDC, Barbosa AS. et al. Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil. Rev Bras Parasitol Vet 2020; 29 DOI: 10.1590/S1984-29612020009.
• 41 Venco L, Manzocchi S, Genchi M. et al. Heat treatment and false-positive heartworm antigen testing in ex vivo parasites and dogs naturally infected by Dirofilaria repens and Angiostrongylus vasorum. Parasit Vectors 2017; 10: 129-134 DOI: 10.1186/s13071-017-2444-6.
• 42 Courtney CH, Zeng Q-Y. Comparison of heartworm antigen test kit performance in dogs having low heartworm burdens. Vet Parasitol 2001; 96: 317-322 DOI: 10.1016/S0304-4017(01)00374-0.
• 43 Stillman BA, Monn M, Liu J. et al. Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs. J Am Vet Med Assoc 2014; 245: 80-86 DOI: 10.2460/javma.245.1.80.
• 44 Rohrbach B, Patton S. Effects of diagnostic test accuracy and treatment efficacy on the occurrence of suspected failure of heartworm prophylaxis in dogs. J Vet Intern Med 2013; 27: 791-797 DOI: 10.1111/jvim.12092.
• 45 Atkins CE. Comparison of results of three commercial heartworm antigen test kits in dogs with low heartworm burdens. J Am Vet Med Assoc 2003; 222: 1221-1223 DOI: 10.2460/javma.2003.222.1221.
• 46 Starkey LA, Bowles JV, Payton ME. et al. Comparative evaluation of commercially available point-of-care heartworm antigen tests using well-characterized canine plasma samples. Parasit Vectors 2017; 10: 145-149 DOI: 10.1186/s13071-017-2447-3.
• 47 Little SE, Munzing C, Heise SR. et al. Pre-treatment with heat facilitates detection of antigen of Dirofilaria immitis in canine samples. Vet Parasitol 2014; 203: 250-252
• 48 McCall JW, Genchi C, Kramer LH. et al. Heartworm disease in animals and humans. Adv Parasitol 2008; 66: 193-285 DOI: 10.1016/S0065-308X(08)00204-2.
• 49 Dressler F, Whalen JA, Reinhardt BN. et al. Western blotting in the serodiagnosis of Lyme disease. J Infect Dis 1993; 167: 392-400 DOI: 10.1093/infdis/167.2.392.
• 50 Engstrom SM, Shoop E, Johnson RC. Immunoblot interpretation criteria for serodiagnosis of early Lyme disease. J Clin Microbiol 1995; 33: 419-427 DOI: 10.1128/jcm.33.2.419-427.1995.
• 51 Barth C, Straubinger RK, Krupka I. et al. Comparison of different diagnostic assays for the detection of Borrelia burgdorferi-specific antibodies in dogs. Vet Clin Pathol 2014; 43: 496-504 DOI: 10.1111/vcp.12213.
• 52 Krupka I, Straubinger RK. Lyme borreliosis in dogs and cats: background, diagnosis, treatment and prevention of infections with Borrelia burgdorferi sensu stricto. Vet Clin North Am Small Anim Pract 2010; 40: 1103-1119 DOI: 10.1016/j.cvsm.2010.07.011.
• 53 Aguero-Rosenfeld ME, Wang G, Schwartz I. et al. Diagnosis of Lyme borreliosis. Clin Microbiol Rev 2005; 18: 484-509 DOI: 10.1128/CMR.18.3.484-509.2005.
• 54 Littman MP, Goldstein RE, Labato MA. et al. ACVIM small animal consensus statement on Lyme disease in dogs: diagnosis, treatment, and prevention. J Vet Intern Med 2006; 20: 422-434 DOI: 10.1111/j.1939-1676.2006.tb02880.x.
• 55 Levy S, Magnarelli L. Relationship between development of antibodies to Borrelia burgdorferi in dogs and the subsequent development of limb/joint borreliosis. J Am Vet Med Assoc 1992; 200: 344-347
• 56 Liang FT, Philipp MT. Analysis of antibody response to invariable regions of VlsE, the variable surface antigen of Borrelia burgdorferi. Infect Immun 1999; 67: 6702-6706 DOI: 10.1128/IAI.67.12.6702-6706.1999.
• 57 Liang FT, Jacobson RH, Straubinger RK. et al. Characterization of a Borrelia burgdorferi VlsE invariable region useful in canine Lyme disease serodiagnosis by enzyme-linked immunosorbent assay. J Clin Microbiol 2000; 38: 4160-4166 DOI: 10.1128/JCM.38.11.4160-4166.2000.
• 58 O'Connor TP, Esty KJ, Hanscom JL. et al. Dogs vaccinated with common Lyme disease vaccines do not respond to IR6, the conserved immunodominant region of the VlsE surface protein of Borrelia burgdorferi. Clin Vaccine Immunol 2004; 11: 458-462 DOI: 10.1128/CDLI.11.3.458-462.2004.
• 59 Philipp MT, Bowers LC, Fawcett PT. et al. Antibody response to IR6, a conserved immunodominant region of the VlsE lipoprotein, wanes rapidly after antibiotic treatment of Borrelia burgdorferi infection in experimental animals and in humans. J Infect Dis 2001; 184: 870-878 DOI: 10.1086/323392.
• 60 Goldstein RE, Beall M, Alleman A. Performance Comparison of SNAP® 4Dx® Plus and AccuPlex® 4 for the Detection of Antibodies to Borrelia burgdorferi and Anaplasma phagocytophilum. Int J Appl Res Vet Med 2014; 12 DOI: 10.1016/j.vetpar.2020.109176.
• 61 Dumler JS, Barbet AF, Bekker C. et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and'HGE agent’as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 2001; 51: 2145-2165 DOI: 10.1099/00207713-51-6-2145.
• 62 Woldehiwet Z. The natural history of Anaplasma phagocytophilum. Vet Parasitol 2010; 167: 108-122 DOI: 10.1016/j.vetpar.2009.09.013.
• 63 Cohn LA. Ehrlichiosis and related infections. Vet Clin North Am Small Anim Pract 2003; 33: 863-884 DOI: 10.1016/S0195-5616(03)00031-7.
• 64 Beaufils J, Inokuma H, Martin Granel J. et al. Anaplasma platys (Ehrlichia platys) infection in a dog in France: description of the case, and characterization of the agent. Rev Med Vet (Toulouse) 2002; 153: 85-90
• 65 Sparagano OA, De Vos AP, Paoletti B. et al. Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. J Vet Diagn Invest 2003; 15: 527-534 DOI: 10.1177/104063870301500604.
• 66 Kontos V, Papadopoulos O, French T. Natural and experimental canine infections with a Greek strain of Ehrlichia platys. Vet Clin Pathol 1991; 20: 101-105 DOI: 10.1111/j.1939-165X.1991.tb00867.x.
• 67 Sainz A, Amusategui I, Tesouro M. Ehrlichia platys infection and disease in dogs in Spain. J Vet Diagn Invest 1999; 11: 382-384 DOI: 10.1177/104063879901100419.
• 68 Harvey JW, Simpson CF, Gaskin JM. Cyclic thrombocytopenia induced by a Rickettsia-like agent in dogs. J Infect Dis 1978; 137: 182-188 DOI: 10.1093/infdis/137.2.182.
• 69 Gaunt S, Beall M, Stillman B. et al. Experimental infection and co-infection of dogs with Anaplasma platys and Ehrlichia canis: hematologic, serologic and molecular findings. Parasit Vectors 2010; 3: 1-10 DOI: 10.1186/1756-3305-3-33.
• 70 Aguirre E, Tesouro M, Ruiz L. et al. Genetic characterization of Anaplasma (Ehrlichia) platys in dogs in Spain. J Vet Med B Infect Dis Vet Public Health 2006; 53: 197-200 DOI: 10.1111/j.1439-0450.2006.00937.x.
• 71 Beall MJ, Chandrashekar R, Eberts MD. et al. Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota. Vector Borne Zoonotic Dis 2008; 8: 455-464 DOI: 10.1089/vbz.2007.0236.
• 72 Foley JE, Foley P, Madigan JE. Spatial distribution of seropositivity to the causative agent of granulocytic ehrlichiosis in dogs in California. Am J Vet Res 2001; 62: 1599-1605 DOI: 10.2460/ajvr.2001.62.1599.
• 73 Carrade D, Foley JE, Borjesson DL. et al. Canine granulocytic anaplasmosis: a review. J Vet Intern Med 2009; 23: 1129-1141 DOI: 10.1111/j.1939-1676.2009.0384.x.
• 74 Eberts MD, Vissotto de Paiva Diniz PP, Beall MJ. et al. Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs. J Am Anim Hosp Assoc 2011; 47: e86-e94 DOI: 10.5326/JAAHA-MS-5578.
• 75 Martin AR, Brown GK, Dunstan RH. et al. Anaplasma platys: an improved PCR for its detection in dogs. Exp Parasitol 2005; 109: 176-180 DOI: 10.1016/j.exppara.2004.11.007.
• 76 Scorpio DG, Dumler JS, Barat NC. et al. Comparative strain analysis of Anaplasma phagocytophilum infection and clinical outcomes in a canine model of granulocytic anaplasmosis. Vector Borne Zoonotic Dis 2011; 11: 223-229 DOI: 10.1089/vbz.2009.0262.
• 77 Egenvall A, Bjöersdorff A, Lilliehöök L. et al. Early manifestations of granulocytic ehrlichiosis in dogs inoculated experimentally with a Swedish Ehrlichia species isolate. Vet Rec 1998; 143: 412-417 DOI: 10.1136/vr.143.15.412.
• 78 Poitout FM, Shinozaki JK, Stockwell PJ. et al. Genetic variants of Anaplasma phagocytophilum infecting dogs in Western Washington State. J Clin Microbiol 2005; 43: 796-801 DOI: 10.1128/JCM.43.2.796-801.2005.
• 79 Lara B, Conan A, Thrall MA. et al. Serologic and molecular diagnosis of Anaplasma platys and Ehrlichia canis infection in dogs in an endemic region. Pathogens 2020; 9: 488
• 80 Zhi N, Ohashi N, Tajima T. et al. Transcript heterogeneity of the p44 multigene family in a human granulocytic ehrlichiosis agent transmitted by ticks. Infect Immun 2002; 70: 1175-1184 DOI: 10.1128/IAI.70.3.1175-1184.2002.
• 81 Barth C, Straubinger RK, Müller E. et al. Comparison of different diagnostic tools for the detection of A naplasma phagocytophilum in dogs. Vet Clin Pathol 2014; 43: 180-184 DOI: 10.1111/vcp.12213.
• 82 Egenvall A, Lilliehöök I, Karlstam E. et al. Detection of granulocytic Ehrlichia species DNA by PCR in persistently infected dogs. Vet Rec 2000; 146: 186-190 DOI: 10.1136/vr.146.7.186.
• 83 Naucke T. Vektor-übertragene Erkrankungen-Borreliose, Babesiose & Co-Relevanz für die deutsche Kleintierpraxis. kleintier konkret 2007; 10: 4-8 DOI: 10.1055/s-2007-967140.
• 84 Mylonakis ME, Koutinas AF, Breitschwerdt EB. et al. Chronic canine ehrlichiosis (Ehrlichia canis): a retrospective study of 19 natural cases. J Am Anim Hosp Assoc 2004; 40: 174-184 DOI: 10.5326/0400174.
• 85 Woody BJ, Hoskins JD. Ehrlichial diseases of dogs. Vet Clin North Am Small Anim Pract 1991; 21: 75-98 DOI: 10.1016/S0195-5616(91)50009-7.
• 86 Ristic M, Huxsoll DL, Weisiger RM. et al. Serological diagnosis of tropical canine pancytopenia by indirect immunofluorescence. Infect Immun 1972; 6: 226-231 DOI: 10.1128/iai.6.3.226-231.1972.
• 87 Buhles WC, Huxsoll DL, Ristic M. Tropical canine pancytopenia: Clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy, and challenge inoculation. J Infect Dis 1974; 130: 357-367 DOI: 10.1093/infdis/130.4.357.
• 88 Bartsch RC, Greene RT. Post-therapy antibody titers in dogs with ehrlichiosis: follow-up study on 68 patients treated primarily with tetracycline and/or doxycycline. J Vet Intern Med 1996; 10: 271-274 DOI: 10.1111/j.1939-1676.1996.tb02061.x.
• 89 Waner T, Harrus S, Jongejan F. et al. Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis. Vet Parasitol 2001; 95: 1-15 DOI: 10.1016/S0304-4017(00)00407-6.
• 90 O'Connor TP, Saucier JM, Daniluk D. et al. Evaluation of peptide-and recombinant protein-based assays for detection of anti-Ehrlichia ewingii antibodies in experimentally and naturally infected dogs. Am J Vet Res 2010; 71: 1195-1200 DOI: 10.2460/ajvr.71.10.1195.
• 91 Chandrashekar R, Daniluk D, Moffitt S. et al. Serologic diagnosis of equine borreliosis: Evaluation of an in-clinic enzyme-linked immunosorbent assay (SNAP (R) 4Dx (R)). Int J Appl Res Vet Med 2008; 6: 145-150
• 92 Beall MJ, Alleman AR, Breitschwerdt EB. et al. Seroprevalence of Ehrlichia canis, Ehrlichia chaffeensis and Ehrlichia ewingii in dogs in North America. Parasit Vectors 2012; 5: 1-11 DOI: 10.1186/1756-3305-5-29.
• 93 Harrus S, Alleman AR, Bark H. et al. Comparison of three enzyme-linked immunosorbant assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia canis. Vet Microbiol 2002; 86: 361-368 DOI: 10.1016/S0378-1135(02)00022-6.
• 94 Liu J, Drexel J, Andrews B. et al. Comparative evaluation of 2 in-clinic assays for vector-borne disease testing in dogs. Top Companion Anim Med 2018; 33: 114-118 DOI: 10.1053/j.tcam.2018.09.003.
• 95 Bélanger M, Sorenson HL, France MK. et al. Comparison of serological detection methods for diagnosis of Ehrlichia canis infections in dogs. J Clin Microbiol 2002; 40: 3506-3508 DOI: 10.1128/JCM.40.9.3506-3508.2002.
• 96 Villanueva-Saz S, Martínez M, Ramirez JD. et al. Evaluation of five different rapid immunochromatographic tests for canine leishmaniosis in Spain. Acta Trop 2022; 229: 106371 DOI: 10.1016/j.actatropica.2022.106371.
• 97 Vlkova M, Rohousova I, Drahota J. et al. Canine antibody response to Phlebotomus perniciosus bites negatively correlates with the risk of Leishmania infantum transmission. PLoS Negl Trop Dis 2011; 5: e1344 DOI: 10.1371/journal.pntd.0001344.
• 98 Živičnjak T, Martinković F, Marinculić A. et al. A seroepidemiologic survey of canine visceral leishmaniosis among apparently healthy dogs in Croatia. Vet Parasitol 2005; 131: 35-43 DOI: 10.1016/j.vetpar.2005.04.036.
• 99 Marty P, Izri A, Ozon C. et al. A century of leishmaniasis in Alpes-Maritimes, France. Ann Trop Med Parasitol 2007; 101: 563-574 DOI: 10.1179/136485907X229121.
• 100 Lazri T, Duscher G, Edelhofer R. et al. Infektionen mit arthropodenübertragenen Parasiten bei Hunden im Kosovo und in Albanien unter besonderer Berücksichtigung der Leishmanieninfektionen. Wien Klin Wochenschr 2008; 120: 54-58 DOI: 10.1007/s00508-008-1076-4.
• 101 Mazeris A, Soteriadou K, Dedet JP. et al. Leishmaniases and the Cyprus paradox. Am J Trop Med Hyg 2010; 82: 441 DOI: 10.4269/ajtmh.2010.09-0282.
• 102 Cortes S, Vaz Y, Neves R. et al. Risk factors for canine leishmaniasis in an endemic Mediterranean region. Vet Parasitol 2012; 189: 189-196 DOI: 10.1016/j.vetpar.2012.04.028.
• 103 Lombardo G, Pennisi MG, Lupo T. et al. Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques. Vet Parasitol 2012; 184: 10-17 DOI: 10.1016/j.vetpar.2011.08.010.
• 104 Miró G, Montoya A, Roura X. et al. Seropositivity rates for agents of canine vector-borne diseases in Spain: a multicentre study. Parasit Vectors 2013; 6: 1-9 DOI: 10.1186/1756-3305-6-117.
• 105 Ntais P, Sifaki-Pistola D, Christodoulou V. et al. Leishmaniases in Greece. Am J Trop Med Hyg 2013; 89: 906 DOI: 10.4269/ajtmh.13-0070.
• 106 Ready P. Leishmaniasis emergence in Europe. Euro Surveill 2010; 15: 19505 DOI: 10.2807/ese.15.10.19505-en.
• 107 Maroli M, Rossi L, Baldelli R. et al. The northward spread of leishmaniasis in Italy: evidence from retrospective and ongoing studies on the canine reservoir and phlebotomine vectors. Trop Med Int Health 2008; 13: 256-264 DOI: 10.1111/j.1365-3156.2007.01998.x.
• 108 Naucke T, Pesson B. Presence of Phlebotomus (Transphlebotomus) mascittii Grassi, 1908 (Diptera: Psychodidae) in Germany. Parasitol Res 2000; 86: 335-336 DOI: 10.1007/s004360050053.
• 109 Naucke T, Schmitt C. Is leishmaniasis becoming endemic in Germany. Int J Med Microbiol 2004; 293: 179 DOI: 10.1016/s1433-1128(04)80036-6.
• 110 Naucke T, Menn B, Massberg D. et al. Sandflies and leishmaniasis in Germany. Parasitol Res 2008; 103: 65-68 DOI: 10.1007/s00436-008-1052-y.
• 111 Solano-Gallego L, Koutinas A, Miró G. et al. Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis. Vet Parasitol 2009; 165: 1-18 DOI: 10.1016/j.vetpar.2009.05.022.
• 112 Solano-Gallego L, Morell P, Arboix M. et al. Prevalence of Leishmania infantum infection in dogs living in an area of canine leishmaniasis endemicity using PCR on several tissues and serology. J Clin Microbiol 2001; 39: 560-563 DOI: 10.1128/JCM.39.2.560-563.2001.
• 113 Quinnell R, Courtenay O, Garcez L. et al. IgG subclass responses in a longitudinal study of canine visceral leishmaniasis. Vet Immunol Immunopathol 2003; 91: 161-168 DOI: 10.1016/S0165-2427(02)00311-2.
• 114 Solano-Gallego L, Llull J, Ramis A. et al. Longitudinal study of dogs living in an area of Spain highly endemic for leishmaniasis by serologic analysis and the leishmanin skin test. Am J Trop Med Hyg 2005; 72: 815-818 DOI: 10.4269/ajtmh.2005.72.815.
• 115 Athanasiou LV, Petanides TA, Chatzis MK. et al. Comparison of two commercial rapid in-clinic serological tests for detection of antibodies against Leishmania spp. in dogs. J Vet Diagn Invest 2014; 26: 286-290 DOI: 10.1177/1040638714523614.
• 116 Ferroglio E, Zanet S, Mignone W. et al. Evaluation of a rapid device for serological diagnosis of Leishmania infantum infection in dogs as an alternative to immunofluorescence assay and Western blotting. Clin Vaccine Immunol 2013; 20: 657-659 DOI: 10.1128/CVI.00719-12.
• 117 Ferroglio E, Centaro E, Mignone W. et al. Evaluation of an ELISA rapid device for the serological diagnosis of Leishmania infantum infection in dog as compared with immunofluorescence assay and Western blot. Vet Parasitol 2007; 144: 162-166 DOI: 10.1016/j.vetpar.2006.09.017.
• 118 Rodríguez-Cortés A, Ojeda A, Todolí F. et al. Performance of commercially available serological diagnostic tests to detect Leishmania infantum infection on experimentally infected dogs. Vet Parasitol 2013; 191: 363-366 DOI: 10.1016/j.vetpar.2012.09.009.
• 119 Villanueva-Saz S, Basurco A, Martín V. et al. Comparison of a qualitative immunochromatographic test with two quantitative serological assays for the detection of antibodies to Leishmania infantum in dogs. Acta Vet Scand 2019; 61: 1-6 DOI: 10.1186/s13028-019-0473-1.
• 120 Solano-Gallego L, Villanueva-Saz S, Carbonell M. et al. Serological diagnosis of canine leishmaniosis: comparison of three commercial ELISA tests (Leiscan^®, ID Screen^® and Leishmania 96^®), a rapid test (Speed Leish K^®) and an in-house IFAT. Parasit Vectors 2014; 7: 1-10 DOI: 10.1186/1756-3305-7-111.
• 121 Iniesta V, Fernández-Cotrina J, Solano-Gallego L. et al. Vaccination with LetiFend^®, a novel canine leishmaniosis vaccine, does not interfere with serological diagnostic tests. In: Proceedings of the X Southern European Veterinary Conference/51º Congreso Nacional Avepa. 2016: 20-22
• 122 Levett PN. Leptospirosis. Clin Microbiol Rev 2001; 14: 296-326 DOI: 10.1128/CMR.14.2.296-326.2001.
• 123 Llewellyn J-R, Krupka-Dyachenko I, Rettinger AL. et al. Urinary shedding of leptospires and presence of Leptospira antibodies in healthy dogs from Upper Bavaria. Berl Munch Tierarztl Wochenschr 2016; 129: 251-257
• 124 Greene CE. Infectious Diseases of the Dog and Cat, 4th edn. St Louis. Missouri: Elsevier; 2012
• 125 Brown P, Gravekamp C, Carrington D. et al. Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. J Med Microbiol 1995; 43: 110-114 DOI: 10.1099/00222615-43-2-110.
• 126 Masri SA, Nguyen PT, Gale SP. et al. A polymerase chain reaction assay for the detection of Leptospira spp. in bovine semen. Can J Vet Res 1997; 61: 15
• 127 Merien F, Amouriaux P, Perolat P. et al. Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J Clin Microbiol 1992; 30: 2219-2224 DOI: 10.1128/jcm.30.9.2219-2224.1992.
• 128 Savio M, Rossi C, Fusi P. et al. Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA. J Clin Microbiol 1994; 32: 935-941 DOI: 10.1128/jcm.32.4.935-941.1994.
• 129 Merien F, Baranton G, Perolat P. Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis. J Infect Dis 1995; 172: 281-285 DOI: 10.1093/infdis/172.1.281.
• 130 Dey S, Madhan MohanC, Ramadass P. et al. Recombinant antigen-based dipstick elisa for the diagnosis of leptospirosis in dogs. Vet Rec 2007; 160: 186-188 DOI: 10.1136/vr.160.6.186.
• 131 Sykes J, Hartmann K, Lunn K. et al. 2010 ACVIM small animal consensus statement on leptospirosis: diagnosis, epidemiology, treatment, and prevention. J Vet Intern Med 2011; 25: 1-13 DOI: 10.1111/j.1939-1676.2010.0654.x.
• 132 Bolin CA. Diagnosis of leptospirosis: a reemerging disease of companion animals. Semin Vet Med Surg Small Anim 1996; 11: 166-171 DOI: 10.1016/s1096-2867(96)80029-6.
• 133 Reagan KL, Sykes JE. Diagnosis of canine leptospirosis. Vet Clin North Am Small Anim Pract 2019; 49: 719-731 DOI: 10.1016/j.cvsm.2019.02.008.
• 134 Levett PN. Leptospirosis: a forgotten zoonosis. ? Clin Appl Immunol Rev 2004; 4: 435-448 DOI: 10.1016/j.cair.2004.08.001.
• 135 Fraune CK, Schweighauser A, Francey T. Evaluation of the diagnostic value of serologic microagglutination testing and a polymerase chain reaction assay for diagnosis of acute leptospirosis in dogs in a referral center. J Am Vet Med Assoc 2013; 242: 1373-1380 DOI: 10.2460/javma.242.10.1373.
• 136 Lizer J, Velineni S, Weber A. et al. Evaluation of 3 serological tests for early detection of leptospira-specific antibodies in experimentally infected dogs. J Vet Intern Med 2018; 32: 201-207 DOI: 10.1111/jvim.14865.
• 137 Langston CE, Heuter KJ. Leptospirosis: A re-emerging zoonotic disease. Vet Clin North Am Small Anim Pract 2003; 33: 791-807 DOI: 10.1016/S0195-5616(03)00026-3.
• 138 Hartman E, Van Houten M, Frik J. et al. Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM-and IgG-specific ELISA. Vet Immunol Immunopathol 1984; 7: 245-254 DOI: 10.1016/0165-2427(84)90083-7.
• 139 Lizer J, Grahlmann M, Hapke H. et al. Evaluation of a rapid IgM detection test for diagnosis of acute leptospirosis in dogs. Vet Rec 2017; 180: 517-517 DOI: 10.1136/vr.104134.
• 140 Kodjo A, Calleja C, Loenser M. et al. A rapid in-clinic test detects acute leptospirosis in dogs with high sensitivity and specificity. Biomed research international 2016; 2016 DOI: 10.1155/2016/3760191.
• 141 Abdoel TH, Houwers DJ, van Dongen AM. et al. Rapid test for the serodiagnosis of acute canine leptospirosis. Vet Microbiol 2011; 150: 211-213 DOI: 10.1016/j.vetmic.2011.01.015.
• 142 Troìa R, Balboni A, Zamagni S. et al. Prospective evaluation of rapid point-of-care tests for the diagnosis of acute leptospirosis in dogs. Vet J 2018; 237: 37-42 DOI: 10.1016/j.tvjl.2018.05.010.
• 143 Gloor CI, Schweighauser A, Francey T. et al. Diagnostic Value of two commercial chromatographic “patient-side” tests in the diagnosis of acute canine leptospirosis. J Small Anim Pract 2017; 58: 154-161 DOI: 10.1111/jsap.12628.