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Horm Metab Res 1979; 11(3): 195-200
DOI: 10.1055/s-0028-1092706
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© Georg Thieme Verlag KG Stuttgart · New York

Studies with Crystalline Insulin from Obese and Lean Mice of the BL/6J Strain

C.  Tober , U.  Krause [*] , J.  Beyer , Lieselotte  Herberg [**]
  • Diabetes Forschungsinstitut an der Universität Düsseldorf und Abteilung für Klinische Endokrinologie an der II. Medizinischen Klinik und Poliklinik der Johannes-Gutenberg-Universität, Mainz, Germany
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Publication History

Publication Date:
17 December 2008 (online)

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Abstract

Crystalline insulin was extracted and purified from the pancreas of obese (BL/6J-ob/ob) and lean mice (BL/6J and BL/6J-ob /+). The two insulin preparations were compared with respect to their radioimmunologic properties as well as their ability to stimulate glucose metabolism in rat epididymal adipocytes and epididymal adipose tissue from obese and lean mice. No significant differences could be seen between the two insulin preparations and thus an insulin of altered biological properties is not likely to be an adequate explanation for the symptoms observed in the obese mouse.

Key words

Obese Mice - Hyperinsulinism - Insulin Sensitivity - Adipose Tissue

1 Supported by Deutsche Forschungsgemeinschaft BE 601/2

2 Supported by Deutsche Forschungsgemeinschaft: SFB Diabetologie/Düsseldorf and Landesamt für Forschung des Landes Nordrhein/Westfalen