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Thromb Haemost 1970; 24(01/02): 248-255
DOI: 10.1055/s-0038-1654231
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Rapid Detection of Fibrinopeptides by Bidimensional Paper Electrophoresis

K Stocker
1   Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland
,
P. W Straub with the technical assistance of
1   Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland
,
B Eckert
1   Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland
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Publication Date:
28 June 2018 (online)

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Summary

A simplified method for the rapid identification of the fibrinopeptides in small amounts of supernatants of fibrin clots is described.

Using this method, we confirmed the earlier observation that Reptilase-R, a thrombin-like enzyme from the venom of Bothrops atrox, exclusively liberates fibrinopeptides A and AP. It was further demonstrated that fibrinopeptide B can be rapidly isolated from small amounts of fibrinogen or even of plasma by thrombin action on washed Reptilase clots.

 

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