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Thromb Haemost 1965; 14(03/04): 445-453
DOI: 10.1055/s-0038-1654881
Originalarbeiten — Original Articles — Travaux Originaux
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The Action on Blood Clotting of Different Lipoid Fractions of Beef Brain* (Methods of Fractionization and Chromatographic Isolation Procedure of a Phospholipid Anticoagulant)

M. J. P Kahn
1   Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles
,
M Hans
1   Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles
,
R. H Bourgain
1   Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles
› Author AffiliationsSupported by a grant from the Fonds de la Recherche scientifique medicale (grant No. 433).
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Publication Date:
24 July 2018 (online)

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Summary

A method of fractionation and isolation of a lipid anticoagulant from beef brain has been described. This method is based on chemical and chromatographic techniques. The lipid anticoagulant was found to be an inositol monophosphatide containing two different fatty acids. The anticoagulant activity has been evidenced in the Russell-viper-venom test and is believed to interfere with the formation of the early intermediate products involving factor X.

 

  • References

  • 1 Chargaff E. Studies on the chemistry of blood coagulation IV-lipid inhibitors of blood clotting occuring in mammalian tissue. J. Biol. 121: 175 1937;
    PubMedGoogle Scholar
  • 2 Tocantins L. M. Antithromboplastic activity of normal and hemophilic plasma. Fed. Proc. 01: 85 1942;
    PubMedGoogle Scholar
  • 3 Tocantins L. M. Demonstration of antithromboplastic activity in normal and hemophilic plasma. Amer. J. Physiol. 139: 265 1943;
    PubMedGoogle Scholar
  • 4 Tocantins L. M. Cephalin, protamin and the antithromboplastic activity of normal and hemophilic plasma. Proc. Soc. exp. Biol. (N. Y.) 54: 94 1943;
    CrossrefPubMedGoogle Scholar
  • 5 Tocantins L. M. Anticephalin activity and the prothrombin conversion rate of normal and hemophilic plasma. Proc. Soc. exp. Biol. (N.Y.) 55: 291 1944;
    CrossrefPubMedGoogle Scholar
  • 6 Overman P. S, Wright I. S. A new blood clotting inhibitor. J. Biol. Chem. 174: 759 1948;
    PubMedGoogle Scholar
  • 7 Hecht E. New inhibitors of first stage of the blood-clotting process. Nature 167: 279 1951;
    PubMedGoogle Scholar
  • 8 Hecht E, Ottens H. Die Bedeutung der Lipoide für Blutgerinnung. Acta haemat. (Basel.) 05: 265 1952;
    PubMedGoogle Scholar
  • 9 Hecht E, Shapiro D. Sphingosine as an inhibitor of blood clotting. Science 125: 1041 1957;
    CrossrefPubMedGoogle Scholar
  • 10 Mushett C. W, Goldsmith DPJ, Kelly K. L. Studies on lipid anticoagulants. J. Biol. Chem. 211: 163 1954;
    PubMedGoogle Scholar
  • 11 Johnson ShA, Seegers W. H. Studies on the plasma defect in hemophilia. Rev. Haematol. 9N° 3 bis: 529 1954;
    PubMedGoogle Scholar
  • 12 Johnson ShA, Seegers W. H. Platelet cofactor I activity in the hemophilia. - Hemophilia and Hemophiloid Diseases. Edt. Brinkhous K. M. Univ. N. Carolina Press; 1957
    Google Scholar
  • 13 Spaet ThHE, Gamier S. Inactivation of Thromboplastin in human blood. J. clin. Invest. 34: 1807 1955;
    CrossrefPubMedGoogle Scholar
  • 14 Silver M. J, Turner D. L, Tocantins L. M. Phospholipid antithromboplastin assay in vitro and evaluation in vivo of purified fractions. Amer. J. Physiol. 190: 8 1957;
    PubMedGoogle Scholar
  • 15 Turner D. D, Silver M. J, Tocantins L. M. Phospholipid antithromboplastin. II Preparation and Properties. Arch. Biochem. Biophys. 77: 249 1958;
    CrossrefPubMedGoogle Scholar
  • 16 Ferguson J. H. Lipoids and blood platelets with references to blood coagulation and the hemorrhagic diseases. Univ. N. Carolina Press; 1960
    Google Scholar
  • 17 Ferguson J. H. Coagulation balance between lipids and lipidinhibitors in the thromboplastic mechanism. Thrombos. Diathes. haemorrh. (Stuttg.) 07: 145 1961;
    PubMedGoogle Scholar
  • 18 Turpini P, Mossa A, Cipolli P. L. La preparazione cromatografica die fosfolipidi su colonna di gel di silico. Bull, della Soc. Ital. Biol. 37: 1317 1961;
    PubMedGoogle Scholar
  • 19 Bourgain P. Action anticoagulante d’un extrait lipoïdique de cerveau de boeuf — C. R. Soc. Biol. 150: 221 1956;
    PubMedGoogle Scholar
  • 20 Bourgain P. Action inhibitrice d’un extrait lipoïdique de cerveau de boeuf vis à vis de la thromboplastine plasmatique. C. R. Soc. Biol. 151: 400 1957;
    PubMedGoogle Scholar
  • 21 Bourgain P. The inhibition of prothrombin activation by a lipoïd extract of beef brain. Arch. int. Physiol. Biol. 67: 100 1959;
    PubMedGoogle Scholar
  • 22 Bourgain R. Etude des propriétés anticoagulantes d’un extrait lipoïdique de cerveau de boeuf. Ed. Acta Medica Belgica, Bruxelles. 1962
    PubMedGoogle Scholar
  • 23 Bourgain R, Kahn K. Etude de l’antagonisme: extrait lipoïdique de cerveau de boeuf/ thromboplastine tissulaire. Arch. int. Physiol. 69: 390 1961;
    PubMedGoogle Scholar
  • 24 Kahn M, Bourgain R. Observations supplémentaires sur l’antagonisme extrait lipoïdique de cerveau de boeuf/thromboplastine plasmatique. Arch. int. Pharmacodyn. 138: 345 1962;
    PubMedGoogle Scholar
  • 25 Folch J. J. Brain cephalin, a mixture of phosphatides : Separation from it of phosphatidylserin, phosphatidyl-etanolamin and a fraction containing an inositol phosphatide. Biol. Chem. 146: 35 1946;
    PubMedGoogle Scholar
  • 26 Folch J. J. The Structure of Phosphatidylserine. J. Biol. Chem. 174: 439 1948;
    PubMedGoogle Scholar
  • 27 Wagner H, Horhammer L, Wolff P. Dünnsichtchromatographie von Phosphatiden und Glycolipiden. Biochem. Z. 334: 175 1961;
    PubMedGoogle Scholar
  • 28 Hanahan D. J, Olley J. N. Chemical nature of monophosphoinositide. J. Biol. Chem. 231: 813 1958;
    PubMedGoogle Scholar
  • 29 Bereblum I, Chain E. An improved method for the colorimetric determination of phosphate. Biol. J. 32: 295 1938;
    PubMedGoogle Scholar
  • 30 Hübscher G, Hawthorne J. N. The isolation of inositolmonophosphate from liver. Biol. J. 67: 523 1957;
    PubMedGoogle Scholar
  • 31 Dole V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J. clin. Invest. 35: 150 1956;
    CrossrefPubMedGoogle Scholar