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Thromb Haemost 1965; 14(03/04): 490-499
DOI: 10.1055/s-0038-1654885
Originalarbeiten — Original Articles — Travaux Originaux
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Neutralization of Antithrombin VI (Fibrinogen Breakdown Products) with Platelet Antiheparin Factor (Platelet Factor 4)*

S Niewiarowski
1   Department of Physiological Chemistry, Medical School, Bialystok, Poland
,
R Farbiszewski
1   Department of Physiological Chemistry, Medical School, Bialystok, Poland
,
A Popławski
1   Department of Physiological Chemistry, Medical School, Bialystok, Poland
› Author AffiliationsAided in part by the grant from Polish Academy of sciences.
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

It has been found that fibrinogen breakdown product – antithrombin VI – is neutralized by the purified preparation of platelet factor 4, obtained by means of zinc acetate precipitation and DEAE chromatography column. It has been suggested that antiheparin activity of platelet factor 4 and its ability to neutralize antithrombin VI may be related to the same protein.

The purified preparation of platelet factor 4 does not influence the fibrinogen – fibrin conversion by thrombin. This means that platelet factor 2 and platelet factor 4 are not the same substance.

Crude platelet extracts neutralize antithrombin III and V. However, the purified product did not interferes with the action of these antithrombins.

 

  • References

  • 1 Bounameaux Y. Thrombine et agglutination plaqnettaire. Arch. int. Physiol. 63: 243 1955;
    PubMedGoogle Scholar
  • 2 Deutsch E, Kain W. In: Johnson S. A, Monto R. W, Rebnek J. W, Horn R. C. Henry Ford Hospital Intern. Symp. Blood Platelets. 337 Detroit 1960, Little Brown; Boston: 1961
    Google Scholar
  • 3 Eiber H. B, Danishefsky I. Heparin in blood. Proc. Soc. exp. Biol. (N. Y.) 94: 801 1957;
    CrossrefPubMedGoogle Scholar
  • 4 Godal H. G. The antiheparin effect of platelets. Scand. J. clin. Invest. 14: 223 1962;
    CrossrefPubMedGoogle Scholar
  • 5 Grette K. Studies on the mechanism of thrombin catalyzed hemostatic reactions in blood platelets. Scandinavian University Books; Oslo: 1962
    Google Scholar
  • 6 Hensen A, Loeliger E. A. Antithrombin III, its metabolism and its function in blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg). suppl. 1, 09..
    PubMedGoogle Scholar
  • 7 Jaques L. B, Monkhouse F. C, Stewart M. A method for the determination of heparin blood. J. Physiol. 109: 41 1949;
    CrossrefPubMedGoogle Scholar
  • 8 Käser-Glanzmann K, Lüscher E. F. The mechanism of platelet aggregation in relation to hemostasis. Thrombos. Diathes. haemorrh. (Stuttg.) 07: 480 1962;
    PubMedGoogle Scholar
  • 9 Kekwick R. A, Mackay M. E, Nance M. H, Record B. R. Purification of human fibrinogen. Biochem. J. 60: 671 1955;
    PubMedGoogle Scholar
  • 10 Kowalski E, Kopec M, Wçgrzynowicz Z. Influence of fibrinogen degradation products (FDP) on platelet agrégation, adhesiveness and viscous metamorphosis. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 406 1964;
    PubMedGoogle Scholar
  • 11 Kowalski E, Budzynski A. Z, Kopeć M, Latallo Z. S, Lipiński B, Wçgrzynowicz Z. Studies on the molecular pathology and pathogenesis of bleeding in severe fibrinolytic states in dogs. Thrombos Diathes. haemorrh. (Stuttg.) 12: 69 1964;
    PubMedGoogle Scholar
  • 12 Lewis J. H, Ferguson J. H. Partial purification of human prothrombin and proconvertin, their characteristics and interaction. J. clin. Invest. 32: 913 1953;
    PubMedGoogle Scholar
  • 13 Loeliger A, Hers JFPh. Chronic antithrombinaemia. (Antithrombin V) with haemorrhagic diathesis in a case of rheumatoid arthritis with hypergammaglobulinaemia. Thrombos. Diathes. haemorrh. (Stuttg.) 01: 499 1957;
    PubMedGoogle Scholar
  • 14 Lüscher E. F. Die Biochemie der Gerinnungsfaktoren der Thrombozyten. Fourth International Congress of Biochemistry. Vienna: 1958: 75 Pergamon Press.;
    Google Scholar
  • 15 Niewiarowski S, Kowalski E. Antithrombin Formation during Proteolysis of Fibrinogen. Vl-th Congress of the European Society of Haematology. 560 Copenhagen; 1957
    Google Scholar
  • 16 Niewiarowski S, Kowalski E, Stachurska J. Influence of fibrinogen derived antithrombin (Antithrombin VI) on the blood coagulation system. Acta Biochim. pol. 06: 43 1959;
    PubMedGoogle Scholar
  • 17 Owren P. A. Blood coagulation and spontaneous hemostasis. XXII International Congress of Physiological Sciences. Leiden; vol. 1 233 1962
    Google Scholar
  • 18 Poplawski A, Niewiarowski S. Dissociation of platelet antiheparin factor (platelet factor 4) from lipoprotein lipase inhibitor. Biochim. biophys. Acta (Amst.) 90: 403 1964;
    CrossrefPubMedGoogle Scholar
  • 19 Poplawski A, Niewiarowski S. Method for determining antiheparin activity of platelets and erythrocytes. Thrombos. Diathes. haemorrh. (Stuttg.) 13: 147 1965;
    PubMedGoogle Scholar
  • 20 Ware A. G, Fahey J. L, Seegers W. H. Platelet extracts, fibrin formation and interaction of purified prothrombin and thromboplastin. Amer. J. Physiol. 154: 140 1948;
    PubMedGoogle Scholar
  • 21 Worowski K, Niewiarowski S, Prokopowicz J. Fibrinolysis and fibrinogen breakdown products (antithrombin VI) in renal venous blood (RVB) in dog. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 87 1964;
    PubMedGoogle Scholar