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Synfacts 2010(6): 0731-0731  
DOI: 10.1055/s-0029-1219900
Polymer-Supported Synthesis
© Georg Thieme Verlag Stuttgart ˙ New York

Direct Solid-Phase Synthesis of the β-Amyloid (1-42) Peptide

Contributor(s): Yasuhiro Uozumi, Aya Tazawa
B. Bacsa, S. Bősze, C. O. Kappe
Karl-Franzens-University Graz, Austria and Eötvös Loránd University, Budapest, Hungary
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Publication History

Publication Date:
20 May 2010 (online)

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Significance

A microwave-assisted solid-phase synthesis of the 42-mer β-amyloid peptide was described. The coupling of Fmoc amino acids (Fmoc AA) with peptide chains attached to a ­ChemMatrix resin (conditions A) followed by deprotection of the Fmoc group under microwave conditions gave the corresponding resin-supported AA peptides. The coupling of Fmoc-His(Trt)-OH was performed at room temperature without microwave irradiation in order to avoid the racemization (conditions B). The desired β-amyloid peptide was obtained via repetition of the protocol within 15 hours overall processing time in 78% crude yield and 83% crude purity.