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Synlett 2014; 25(10): 1345-1349
DOI: 10.1055/s-0033-1341024
DOI: 10.1055/s-0033-1341024
synpacts
Upgrading Nature’s Tools: Expression Enhancement and Preparative Utility of the Halogenase RebH
Further Information
Publication History
Received: 07 December 2013
Accepted after revision: 25 February 2014
Publication Date:
03 April 2014 (online)
Abstract
Enzymes often excel at performing green, selective chemical reactions, and thus offer key advantages over many chemocatalytic processes. We recently reported the enhanced expression of a regioselective halogenase, RebH, in E. coli, as well as its use in the preparative halogenation of a range of substrates. These improvements have made it possible to begin directed evolution to improve further the activity and substrate scope of RebH. Chemistry is in need of a regioselective halogenation catalyst, and this work illustrates the potential and recent gains made towards using enzymes for this goal.
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