Upgrading Nature’s Tools: Expression Enhancement and Preparative Utility of the Halogenase RebH

Abstract

Enzymes often excel at performing green, selective chemical reactions, and thus offer key advantages over many chemocatalytic processes. We recently reported the enhanced expression of a regioselective halogenase, RebH, in E. coli, as well as its use in the preparative halogenation of a range of substrates. These improvements have made it possible to begin directed evolution to improve further the activity and substrate scope of RebH. Chemistry is in need of a regioselective halogenation catalyst, and this work illustrates the potential and recent gains made towards using enzymes for this goal.

Key words

halogenation - catalysis - enzymes - directed evolution - protein engineering

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