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Vet Comp Orthop Traumatol 2016; 29(01): 14-19
DOI: 10.3415/VCOT-15-03-0046
Original Research
Schattauer GmbH

Assessment of canine autologous platelet-rich plasma produced with a commercial centrifugation and platelet recovery kit

Chris W. Frye
1   Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
,
Andrew Enders
1   Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
,
Marjory B. Brooks
2   Department of Population Medicine, Cornell University College of Veterinary Medicine, Ithaca, NY USA
,
Angela M. Struble
1   Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
,
Joseph J. Wakshlag
1   Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
› Author Affiliations
Further Information

Publication History

Received: 17 March 2015

Accepted: 24 July 2015

Publication Date:
19 December 2017 (online)

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Summary

Objectives: To characterize the cellular composition (platelets, erythrocytes, and leukocytes) and confirm reproducibility of platelet enrichment, as well as determine the platelet activation status in the final product of a commercial platelet-rich plasma kit using canine blood.

Methods: Venous blood from 20 sedated client-owned dogs was used to prepare platelet-rich plasma (PRP) from a commercial kit. Complete blood counts were performed to determine erythrocyte, leukocyte, and platelet numbers in both whole blood (WB) and resultant PRP. The WB and PRP samples from jugular (fast collection) and cephalic (slow collection) venipuncture were also compared. P-selectin externalization was measured in WB and PRP samples from 15 of 20 dogs.

Results: This commercial kit produced an average percent recovery in platelets of 64.7 ± 17.4; erythrocytes of 3.7 ± 0.8, and leukocytes of 31.6 ± 10.0. Neutrophil, monocyte, and lymphocyte percent recovery was 19.6 ± 7.2, 44.89 ± 19.8, and 57.5 ± 10.6, respectively. The recovery of platelets from jugular venipuncture (59.7 ± 13.6%) was lower than from cephalic recovery (68.8 ± 19.1%). The mean percent P-Selectin externalization for WB, PRP, and PRP with thrombin was 25.5 ± 30.9, 4.5 ± 6.4, and 90.6 ± 4.4 respectively.

Clinical significance: Cellular reproducibility of this kit was confirmed and platelets were concentrated within autologous serum. Additionally, measurements of P-selectin externalization showed that platelets are inactive in PRP unless stimulated to degranulate.

Keywords

Platelet-rich plasma - PRP - P-selectin - platelet activation - leukocytes - canine
 

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